ZW57

0. WASH YOUR GODDAMN HANDS.

We're not working in a lab here, cross-conatamination WILL happen. Your job is to minimize it as much as possible. If you end up in a place where soap hasn't been invented yet, wash your hands in distilled alcohol. Your skin won't thank you, but you can afford all the nice hand creams after you cure the plague and get rich.

Find some Penicillium mushrooms!

Yes, penicilin is produced by mushrooms, though Ascomycotes are usually called moulds, it's a fungus, and it makes me laugh to call it a mushroom. Plus, in the middle ages, mushrooms were known to have medicinal properties, so you'll get a lot farther by calling them mushrooms rather than molds.

First thing you need: mouldy fruit. Oranges, or cantaloupes are preferred.

Here's the thing: mold is everywhere, so getting it will be the easiest part. The tricky part start with identifying the correct mold. You don't want to feed your patients black mold, do you?

So. Leave some fruit out. The more the better, because you want to up your chances. Then let it rot in warm and humid places. After a while, pick any fruit that looks white on the outside and green in the middle:

Not the best picture, but that's what it should look like.

2. Transplanting your (potential) Penicillium mushrooms

Until you get it on a plate it's damn near impossible to tell which mold you got. Get ready for some trial and error because you will have to sift through a lot of unwanted mold. You might want to wear a mask.

First you need something to transplant it onto. Making modern agar plates is probably impossible but thankfully not needed. You just need:

Glass plates (the kind that can be closed, you want to minimize cross contamination)

1-2 cup of Hot water (preferably distilled, ask your alchemist if he can do that)

1 cup whole milk (should be 13g of lactose per cup, if your Penicillium won't grow adjust the water-milk ration in favor of milk)

If available: Instead of milk use corn steep liquor. Unfortunately only available after America was discovered, so YMMW, but Penicillium LOVES this stuff. It will make your life SO much easier if it's available.

Pinch of salt

1 teaspoon Yeast extract (get it from a baker)

3-6 teaspoons Gelatin (get it from a butcher)

Disclaimer: The ratio of each of the ingredients will have to be adjusted depending on the purity of the ingredients and on the conventional measuring sizes of the place you end up.

Gently mix it all in and pour out into the plates, let it solidify. If you end up dumped far enough that such refinement isn't possible, make bone broth and strain it through cheesecloth several times to make it as clear as possible, then mix it 5/6 broth and 1/6 milk. Again, if available, use corn steep liquor, but if not milk is fine. Add gelatin (should still be able to get it from the butcher) as needed to solidify it. I'm afraid experimentation will be needed depending on the resources you will be working with.

When you're done, you should have something like this:

Now that you have your plates, run an inoculation loop through a flame to sterilize it.

Something like this. Wave it through the air to cool it so you don't kill your mold, grab it from your fruit and geeeeeently spread it on top of your improvised agar without breaking the surface of the gelatin!

You can see the motions on this one pretty well. Close your plates, stack them about a meter/3ft from the fireplace. Judge for yourself, but ideally somewhere you would consider comfortably warm (20-24°C).

3. Identifying your Penicillium Mushrooms

If all went well, you are going to have something that looks like this:

Well, realistically, it will look something like this:

We're not actually doing it in a lab, after all. But IDEALLY, it will look like the above. It doesn't have to be perfect, you just need to be able to identify Penicillium molds for now.

IDEALLY, on the plate that matches the description of the penicillium mold you'll see an exclusion zone of bacteria around the mold, like the fourth plate in the second row, so you know you have a potential winner, but if you managed to avoid bacterial growth you need to take a few extra steps.

Penicillium molds have characteristic rings of growth, grey-green-white rings. They're easy to differentiate from bacteria because the molds are fuzzy and the bacteria as smooth and slimy. In the above picture, there are four plates that potentially have what we want, and two are less certain than others. Wash out the unwanted ones, make new agar plates, sterilize your inoculation loop and transplant your best candidates. You might need to do this several times.

Two types are confirmed to produce penicilin: P. chrysogenum and P. rubens.

The former is far more widely used today, but since we're sourcing them from literally thin air, we're more likely to get P. rubens, but unless you're a mycologist you probably won't be able to tell the difference. Thankfully you won't need to, because they both produce penicillin. Which brings me to the next step.

4. Confirming it's the penicillin producing mushroom

We're gonna need more agar plates for this one, and believe it or not, you're gonna need to mix blood into your agar. Wash your hands THROUGHLY.

(Theoretically you can get away with just milk, but identifying the correct bacterial colony on white agar is going to be a nightmare, so just add some sheep blood to your agar, conventionally it's about 5% by volume but you might need more to make it)

You need some gram-positive bacteria, preferably of the Bacillota type. Please don't go out and find a patient with fucking botulism or tetanus, you need to live long enough to make the cure. Instead, if you have a vagina, scrape some of the white, mucousy stuff from there and plant it on your plate. If you don't have your own vagina, a borrowed one is fine. Penicilin also works on Treponema pallidum, so if you get a syphilis-affected prostitute that should also work. Just wear gloves.

Ideally you get something like this.

This is actually Lactobacillus brevis, but Lactobacillus colonies all look relatively the same. The important thing is that it's all gram-positive, and will therefore be affected by penicillin.

Take new plates again, plant your Penicillium mold in the middle, and the bacteria all around it, getting as close to the center as possible. You can put down a paper marker for the mold. Wait for about 20 days.

Ideally, on at least one plate, you will get something like this:

This is literally a textbook example of testing antibiotics, but the Zone of Inhibition is what you're looking for. It means the mold is releasing a compound to kill the competing bacteria for resources, in this case, Beta-lactam antibiotic, or penicillin. Make sure to pick the one with the WIDEST ZoI, because that's the one that produces most penicillin.

So now we have the root stock, but our problems have just begun. This is the part where you're absolutely going to need an alchemist's help.

The problem is that a human body is not a petri dish. It's quite a bit larger. And you want the good bacteria destroying stuff without all the nasty contaminants, so you need a SHITLOAD of mold producing a LOT of penicillin, and then you need a way to filter it. You are going to need actual lab equipment for that, or near as they had it.

Jesus screamed “JESUS CHRIST YOU STABBED ME”.

Since that Jesus had to be taken down due to a bad case of stab-itis, the backup Jesus came in, but he weighed significantly less than the original Jesus- which would have been fine, except that at the end the cross was supposed to ascend upwards with Jesus on it, and the weights hadn’t been adjusted.

my first year doing this, our industry contacts were 1 actor, 1 casting director, and 1 producer to get different aspects of the business, and they all gave us amazing advice and told fantastic stories. the actor in question was Zazu on Broadway’s The Lion King for several years, and told the best story by far.

in The Lion King, there are only two pieces of pre-recorded noise in the whole show. one, when Pumbaa does a MASSIVE fart while fighting the hyenas, and the other being Mufasa saying REMEMBERRRRRR as Simba climbs Pride Rock. the actor told us while struggling not to laugh that, during one night’s performance, someone forgot to flip the tape of these pre-recorded noises.

so, at the end of the show, the great climax where Simba finally accepts his place in the Circle of Life, the heavens parted and-

PFFFFFFFFFRRRRRBTFTBTBFTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT

everyone froze. and then all ran off stage positively HOWLING with laughter.

Originally, the actors were going to stroll on stage with our Lefou hauling in the really neat (and real!) taxidermied deer head that we had found in a local thrift store. Now, two days before opening night, our director wants Lefou to run in from off stage and catch a stuffed duck that Gaston has just shot. This, of course, requires two things to work properly as a scene: a gunshot noise, and a stuffed duck.

The gunshot noise, we had covered. Blue-collar, redneck school? Guns a plenty to record. The stuffed duck? Harder than you might have thought to obtain.

Three hunting stores, two taxidermists, and one Pet Supply Store ™, I’d finally found a semi-realistic pheasant squeaky toy. What follows is an account of the ways this dog toy managed to be the nightmare prop of the six show run.

Opening Night: The stagehand, who was supposed to drop the bird from the ceiling catwalk, missed his cue and didn’t drop the it. Lefou’s actor rolls with it and does an excellent job of looking around foolishly before getting cuffed upside the head by Gaston. The stagehand then drops the bird squarely on Gaston’s head. Cue laughter.

Saturday Matinee: Different stagehand throws the bird instead of dropping it and beans Lefou directly in the face with the prop. Lefou falls over. Cue laughter.

Saturday Night: Bird is missing during curtain call. Director hauls the deer head down from it’s place on the tavern wall and tells Gaston and Lefou to revert to the old blocking i.e. no gunshot, no bird, just walk in with trophy. During Gaston and Lefou’s conversation, gun shot sound goes off and a stagehand throws the bird onto the stage…from the wrong side of the stage. Lefou and Gaston stare at it in awkward silence for a solid thirty seconds before Lefou makes off-script, subtle joke about Gaston’s gun going off late instead of early. Cue adults in the audience laughing.

Sunday Matinee: Director begs the stagehands to get the cue right at least once. Gunshot and bird prop go off without a hitch. Lefou accidentally catches the prop when it falls from the catwalk. He’s so startled that he caught it that Gaston runs right in to him. They drop both the gun and the bird props, and grab the wrong prop in their scramble. Gaston spends the rest of the scene gesturing dramatically with a stuffed pheasant, instead of a gun.

Sunday Night:  Director is fed up with bird prop, decides that Lefou should just carry bird prop in after gunshot happens off stage. Lefou accidentally squeezes the prop during the intro conversation, startling both actors into silence with the squeaky toy noise - apparently, neither of them realized it was a dog toy.

It was awesome because when people saw the play and didn’t know the girls were identical twins, it literally looked like it was one actor doing multiple, uber fast costume changes.

One of our first performances was for our peers and it was a big school so lots of people didn’t know the twins. This - for some reason - was also the performance they chose to record.

Listening to the confusion of the audience during the playback was fantastic and completely topped by the moment Viola walked off stage left just as Sebastian walked on stage right and someone right beside the camera goes “OH WHAT THE FUCK” so loudly it drowned out everything else.

It’s like our third show and everything has been going surprisingly well. Hook and Smee climb to the top of the “tree trunk”, supposedly looking for Peter and not knowing they’re standing above his hiding spot the whole time.

Turns out someone didn’t close the trapdoor properly, because the second Hook steps on it, he plunges through the thing. He’s able to catch himself, but he’s got his ass and one leg dangling through this hole where it’s like a ten foot drop to the ground. All of us stage crew are literally two feet away from him offstage, just gaping at him because???? Y'all this fall looked BAD. Looked like my dude did the splits in mid air. The whiplash caused his fucking wig to come off. The audience is dead silent, all of us backstage are dead silent, the director is like already looking up how to treat a broken groin.

I love klezmer.

But as a former band kid, this made me die laughing.

"oh ryland. only you would cry over a bunch of rocks."

Enter my mostly black demon who likes to jump out of dark spaces at me.

Movement out of the corner of your eye? Cat.

Objects moved? Cat.

Inexplicable smells? Cat.

Corpses (small)? Cat.

go away? Nah. They just make

you stop being scared.

I was so taken by the image of 20-30 pebbles activating in unison that I had to draw it immediately

Right is the color On ops Blog

Left is how it appeared on my dash

human: here is birds in water