Lurking towards Bethlehem

0. WASH YOUR GODDAMN HANDS.

We're not working in a lab here, cross-conatamination WILL happen. Your job is to minimize it as much as possible. If you end up in a place where soap hasn't been invented yet, wash your hands in distilled alcohol. Your skin won't thank you, but you can afford all the nice hand creams after you cure the plague and get rich.

Find some Penicillium mushrooms!

Yes, penicilin is produced by mushrooms, though Ascomycotes are usually called moulds, it's a fungus, and it makes me laugh to call it a mushroom. Plus, in the middle ages, mushrooms were known to have medicinal properties, so you'll get a lot farther by calling them mushrooms rather than molds.

First thing you need: mouldy fruit. Oranges, or cantaloupes are preferred.

Here's the thing: mold is everywhere, so getting it will be the easiest part. The tricky part start with identifying the correct mold. You don't want to feed your patients black mold, do you?

So. Leave some fruit out. The more the better, because you want to up your chances. Then let it rot in warm and humid places. After a while, pick any fruit that looks white on the outside and green in the middle:

Not the best picture, but that's what it should look like.

2. Transplanting your (potential) Penicillium mushrooms

Until you get it on a plate it's damn near impossible to tell which mold you got. Get ready for some trial and error because you will have to sift through a lot of unwanted mold. You might want to wear a mask.

First you need something to transplant it onto. Making modern agar plates is probably impossible but thankfully not needed. You just need:

Glass plates (the kind that can be closed, you want to minimize cross contamination)

1-2 cup of Hot water (preferably distilled, ask your alchemist if he can do that)

1 cup whole milk (should be 13g of lactose per cup, if your Penicillium won't grow adjust the water-milk ration in favor of milk)

If available: Instead of milk use corn steep liquor. Unfortunately only available after America was discovered, so YMMW, but Penicillium LOVES this stuff. It will make your life SO much easier if it's available.

Pinch of salt

1 teaspoon Yeast extract (get it from a baker)

3-6 teaspoons Gelatin (get it from a butcher)

Disclaimer: The ratio of each of the ingredients will have to be adjusted depending on the purity of the ingredients and on the conventional measuring sizes of the place you end up.

Gently mix it all in and pour out into the plates, let it solidify. If you end up dumped far enough that such refinement isn't possible, make bone broth and strain it through cheesecloth several times to make it as clear as possible, then mix it 5/6 broth and 1/6 milk. Again, if available, use corn steep liquor, but if not milk is fine. Add gelatin (should still be able to get it from the butcher) as needed to solidify it. I'm afraid experimentation will be needed depending on the resources you will be working with.

When you're done, you should have something like this:

Now that you have your plates, run an inoculation loop through a flame to sterilize it.

Something like this. Wave it through the air to cool it so you don't kill your mold, grab it from your fruit and geeeeeently spread it on top of your improvised agar without breaking the surface of the gelatin!

You can see the motions on this one pretty well. Close your plates, stack them about a meter/3ft from the fireplace. Judge for yourself, but ideally somewhere you would consider comfortably warm (20-24°C).

3. Identifying your Penicillium Mushrooms

If all went well, you are going to have something that looks like this:

Well, realistically, it will look something like this:

We're not actually doing it in a lab, after all. But IDEALLY, it will look like the above. It doesn't have to be perfect, you just need to be able to identify Penicillium molds for now.

IDEALLY, on the plate that matches the description of the penicillium mold you'll see an exclusion zone of bacteria around the mold, like the fourth plate in the second row, so you know you have a potential winner, but if you managed to avoid bacterial growth you need to take a few extra steps.

Penicillium molds have characteristic rings of growth, grey-green-white rings. They're easy to differentiate from bacteria because the molds are fuzzy and the bacteria as smooth and slimy. In the above picture, there are four plates that potentially have what we want, and two are less certain than others. Wash out the unwanted ones, make new agar plates, sterilize your inoculation loop and transplant your best candidates. You might need to do this several times.

Two types are confirmed to produce penicilin: P. chrysogenum and P. rubens.

The former is far more widely used today, but since we're sourcing them from literally thin air, we're more likely to get P. rubens, but unless you're a mycologist you probably won't be able to tell the difference. Thankfully you won't need to, because they both produce penicillin. Which brings me to the next step.

4. Confirming it's the penicillin producing mushroom

We're gonna need more agar plates for this one, and believe it or not, you're gonna need to mix blood into your agar. Wash your hands THROUGHLY.

(Theoretically you can get away with just milk, but identifying the correct bacterial colony on white agar is going to be a nightmare, so just add some sheep blood to your agar, conventionally it's about 5% by volume but you might need more to make it)

You need some gram-positive bacteria, preferably of the Bacillota type. Please don't go out and find a patient with fucking botulism or tetanus, you need to live long enough to make the cure. Instead, if you have a vagina, scrape some of the white, mucousy stuff from there and plant it on your plate. If you don't have your own vagina, a borrowed one is fine. Penicilin also works on Treponema pallidum, so if you get a syphilis-affected prostitute that should also work. Just wear gloves.

Ideally you get something like this.

This is actually Lactobacillus brevis, but Lactobacillus colonies all look relatively the same. The important thing is that it's all gram-positive, and will therefore be affected by penicillin.

Take new plates again, plant your Penicillium mold in the middle, and the bacteria all around it, getting as close to the center as possible. You can put down a paper marker for the mold. Wait for about 20 days.

Ideally, on at least one plate, you will get something like this:

This is literally a textbook example of testing antibiotics, but the Zone of Inhibition is what you're looking for. It means the mold is releasing a compound to kill the competing bacteria for resources, in this case, Beta-lactam antibiotic, or penicillin. Make sure to pick the one with the WIDEST ZoI, because that's the one that produces most penicillin.

So now we have the root stock, but our problems have just begun. This is the part where you're absolutely going to need an alchemist's help.

The problem is that a human body is not a petri dish. It's quite a bit larger. And you want the good bacteria destroying stuff without all the nasty contaminants, so you need a SHITLOAD of mold producing a LOT of penicillin, and then you need a way to filter it. You are going to need actual lab equipment for that, or near as they had it.

Downstairs - Frank Gregory , 2022.

American, b. 1962 -  

Very much did in the sense that I literally do not know where my job is at the moment. But, for the time being I haven't been let go because nobody else including the store owner knows where it is either.

So, I don't wanna risk doxxing myself by posting pictures but goddamn am I tempted because this is not a believable event. This is a cartoon problem. For looneytoons.

But yeah, so, I work(ed?) at a kiosk selling boba tea, right? Freestanding kiosk in the mall with full water and electrical hookups and multiple fridges and sinks and a mini kitchen and the works. Fully functional tea shop. Very important to note that it was there last night, The work chat was discussing another issue last night at closing time. I'll get back to this.

It's been showing signs of being on the way out with how business is being handled lately and I've been considering other options, which is probably why I'm not as torn up about this as I should be, but maybe it just hasn't set in yet, but that's not the point. The point is there's been a lot of shit breaking and not being replaced and nobody mentioning anything about it until I walk into work in the morning and have to figure out why shit like the fucking cash register isn't there today. So I'm kinda used to having to ask questions about big things that nobody bothered to update me on. I was out for two weeks recovering from a surgery, so I came to work this morning assuming there'd be some kind of bullshit, yeah?

So, the question I had to ask the chat this morning was:

Not a text I ever thought I'd have to send in sincerity, but there it is. Because what I found instead was a fenced off patch of discolored tiles and a few holes in the floor where my entire place of employment used to be.

And the answer? Nobody knows! It was there last night when the mall closed, and every single trace of the structure and all its contents including drink making supplies and our safe and cashbox was gone when it opened again. And when I say nobody knows, I mean everyone from last night's closers to the actual (former?) owner of the store jad no fucking clue about this until getting that text from me this morning. For once I am actually the first to know. 🎉.

So. I guess I didn't so much lose my job as had it stolen. Not by AI, but good old fashioned hands-on human beings picking it up and carrying it away somehow. All mall security would tell me was that they were instructed not to tell me anything and have us contact our management. Who also don't know anything. And later on I came across some construction workers around the gravesite of the kiosk discussing filling in the holes, asked them about it, and was told that they "weren't at liberty to say".

So, not only is my job gone in the most literal physical sense of the word, but it was taken in some kind of super secret kiosk extraction in the dead of night without any warning or witnesses and nobody is allowed to speak of it. The store owner said she was gonna figure it out 10 hours ago and still no word back.

My mother in law thinks the FBI took it.

Not any of the other stores around the state. Just the one little kiosk.

Why? Because she loves a conspiracy and is just a little bit extra.

Also because she was around for the massive crackdown on Yakuza-owned businesses in Waikiki (in her homestate) that did actually involve the FBI seizing stores (no confirmation of making kiosks cleanly disappear in the middle of the night though).

Still no word from my job on what's actually going on, but the most likely theory so far is that maybe the kiosk was on lease and got repossessed? The mystery continues

According to the owner, based on what she's been able to find out, the kiosk was not removed legally and they're starting a potentially long process of legal action. I hope she gets to sue the shit out of whoever did it but for now at least I know for sure I'm unemployed.

Really hoping for more details in terms of who/why/how, so I'll keep updating if I learn anything.

For now the summary is: An unnamed entity that is most likely mall management (on account of mall security cooperating with them) stole an entire kiosk and all the contents including money and machinery with barely a trace in the middle of the night grinch-style, with zero warning or explanation, and ensured the silence of both security and the construction crew, in an action that was definitely preplanned and illegal, and as far as I know nobody knows its whereabouts.

Enjoy the unintentionally funniest text I've ever sent in my life

Aaand a close-up:

HEY HI HELLO THIS ONE'S MY FAVORITE

apparently, cats' kidneys tend to be the thing that takes them down, something about their bodies being unable to self-clean their kidneys, and the vaccine is supposed revitalize the body's ability to do just that. It would be very VERY fucking cool to have cats suddenly reaching 30 years of age be the normal thing.

dude cracked the case about WHY kidneys fail, across the board as far as i can tell. turns out there's a specific molecule whose job it is to attach to waste and signal macrophages to come eat it. it remains inactive in cats for some reason, but the molecule is still there. basically what he's done is found the switch to activate them. this will be profound not only for our domestic babies, but for big cats too - especially cheetahs!

although his research was focused on cats, it's already being used to develop drugs for humans too!

on top of that, since these molecules are tags for waste, this could also dramatically lower the rate of fatty liver disease, liver cancer, urinary crystals, rheumatoid arthritis, and even some neurological cases! like, they're hoping it may have an impact on parkinson's and alzheimers, but it DOES have an impact on stroke recovery. like. holy shit.

furthermore, he's insisting that the feline drug be affordable if and when it rolls out onto the market. he wants this to be something anyone can get for their cat!! idk how much sway he'll have over the human drug, but hopefully enough that it, too, won't be that expensive.

annnnnd in his research that he's still doing for the human side of things, he's found a potential link between this molecule and estrogen. in the 20,000 samples he's tested, women between ages 10 and 29 had the highest amount of this molecule present in their blood (a higher amount means Something Fucky is going on, essentially. There's a higher amount of waste the body is trying to clean out) but it drops down to be almost equal amongst men and women after menopause. it hasn't been looked into yet, but fuck, just the fact it's noted and known and probably WILL be looked into soon??? imagine if this is what leads to figuring out all the various ways the ovaries and uterus fucks with people and how to fix it. or even like, maybe there's something about estrogen that makes it work better. who knows! but it's rad the link is there to be researched :D

As for the study itself, the 360 day follow up on stage 3 kidney failure kitties showed that the control had a survival rate of about 20%, while the test group had a survival rate of 80%

earlier this week Twitter user ppuccin0 tweeted about a fashion article that advised against tops with large floral patterns, saying the wearer was in danger of looking like a "ロマンティックおばさん," or a "romantic auntie." the tweet went viral with many agreeing that a "romantic auntie" sounded like a very nice thing to aspire to be, and some even posted illustrations or photos tagged with the trend

illustration by Toyota Yuu (author of Cherry Magic)

illustration by 141shkw/Sora Midori (author of Beautiful Curse)

photos by Takinami Yukari (author of Motokare Mania and Watashi-tachi wa Mutsuu Ren'ai ga Shitai or "We Want A Painless Romance")

illustration by m:m (mangaka of Matataki no End Roll)

illustration by ooinuai (mangaka of Onikui Kitan)

illustration by ma2 (mangaka of The Reason We Fall In Love)

BONUS:

A Koa'e Kea flies over a lava fountain during a volcanic eruption in Hawaii. This seabird forages over tropical seas and has distinctive long tail streamers.

Lava fountains form when gas escapes from rising magma, sending molten rock high into the air.

After a few hours, I didn’t hear back from them so I went home. My parents were home and had stacked a bunch of groceries on top of the letter without opening it. They said “hi” and I said “hi” and went down stairs to the basement. I held my dog and panicked about what to do. My sister, who knew that I had written them a letter of great importance, told me they hadn’t read it yet. She also told me she could ask them to do so. I consented to this and stayed in the basement. A few minutes later my dad knocked on the door and poked his soft smooth little nerd head in and said “hey buddy” and I started crying so hard I almost vomited. He came over and gave me a BIG hug and said that it was gonna be OK, he was OK with this, he knew it must have been hard but he was here for me. He told me he and my mom had already talked years before they had me about how if they had to pick between their faith and their child they’d pick their child. It was a very sweet moment. I came out to my mom later that evening and we were both bawling the whole time.

The day after I came out to my parents, I came out to my brother @inbabylontheywept at a Mexican restaurant and he took it like a champ. That evening my mom took me for a walk and looked almost angry - she said she wanted to make sure that I didn’t use being a woman as an excuse to not go to grad school. I told her I wouldn’t and she instantly looked relieved and happier.

My dad, on the other hand, seemed to struggle with it. He kept asking me if I had a boyfriend, and I told him I did not. He kept asking me if I wanted to go clothes shopping with him and I did not. He kept asking me if I would let him go to some of my shows, and I had NO idea what he was talking about.

Finally, 6 months after coming out, of awkward misgendering and questions that didn’t make sense from my dad, he excitedly pokes his soft smooth little nerd head into my bedroom again and says “I found a movie about Your People.” My people. I was absolutely bewildered, but he was so excited and I knew he had been trying SO hard so I watched it with him. It was The Birdcage, and it was amazing. It also was revelatory in that I finally realized why my initially-supportive father seemed to be having such a hard time with my pronouns and stuff - he didn’t know what the difference between trans and doing drag was. After the movie he again asked if I would invite him to one of my shows, and I said, “Hey dad, you know how about half the world is women?” And he said “yeah,” and I said “Well, see, I’m on that half now. I’m not doing drag.” And it was like a switch flipped in his brain. He was like “omg that’s so easy? I was so confused about what to call you when?”