Jul 1, 2020
Review the role of glucokinase in liver and pancreatic cells!
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Review the role of glucokinase in liver and pancreatic cells!
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Review the kinetics of hexokinase and glucokinase!Â
HEXOKINASE (I, II & III) vs. GLUCOKINASE (IV) Tissue distribution * Hexokinase - Skeletal muscle (most common example even though it occurs throughout most of the body) * Glucokinase - The liver - The pancreas (its beta cells) - Less notably,
Review kinetics of glucokinase and heokinase!Â
Regulators
Hexokinase is regulated by its products: ADP and glucose-6-phosphate. Hexokinase is allosterically inhibited by glucose-6-phosphate.
Glucokinase is activated by un-phosphorylated phosphofructokinase 2 and fructose-2,6-bisphosphate. They keep glucokinase in the cytoplasm and active.
Phosphofructokinase is inhibited by ATP and citrate. (See 10/10/18 lecture at around 20 minutes). Phosphofructokinase is activated by ADP, AMP (AKA adenylate), and fructose-2,6-bisphosphate.
Pyruvate kinase is inhibited allosterically by ATP. Pyruvate kinase is inhibited by alanine. Pyruvate kinase is inhibited by phosphorylation (using PKA). Pyruvate kinase is dependent on lots of fructose-1,6-bisphosphate (pyruvate kinase can “see” what phosphofructokinase is doing; if phosphofructokinase is v. active and making a lot of fructose 1,6-bisphosphate, that’s “telling” pyruvate kinase to keep making pyruvate, which you don’t want if you’re trying to inhibit pyruvate kinase)Â
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echo4charlie replied to your post: A glimmer of hope....Today I choose to let the good outweigh the bad. what are you researching? English please. :P
I work on an enzyme called Glucokinase which is involved in the first step of glucose metabolism in the pancreas and liver. It has a lot of applications for diabetes therapeutics. I study the structural and mechanistic aspects of glucokinase.
Say hello to my bitch.
Glucokinase you silly bitch....