Honestly, I don't think I can say that it's just some characters in Nikke that have transfem vibes, fighting against transmisogyny and its effects on society is an integral part of Nikke's story on a fundamental level.
The story is, at its core, about a demographic of women who are othered, discriminated, hated, and objectified just because of who they are. A point is made that only women make up this group. They are simultaneously expected to perform both femininity and masculinity, yet their efforts are not recognized, ignored, or attributed to others. Most of them are immediately "clockable" for what they are, with only a select few able to "pass" as "real" humans. Society at large views them as repulsive and disgusting, but also as sex objects that exist to be used and discarded.
The women in this othered group are treated as machines, objects, and tools, yet are undeniably still human beings with all the same feelings and needs. Like it's emphasized that, for all intents and purposes, they are indistinguishable from normal people but are nonetheless recognized as "the other" simply for their nature. There are in universe slurs for them, there are major plot beats about the hate crimes committed against them.
These women are viewed as disposable, and their independence and rights are seen as inherently violent. Systems are in place to force them into obedience and compliance, and society at large sees any threat to those systems as impending doom. The state labels anyone that advocates for the rights of these othered women as a criminal.
Their lives are colorful and varied, each one unique and beautiful, but all have one thing in common: they were all someone else before becoming the person they are now. Their accounts and feelings about this vary, from seeing their "old self" as an extension of who they are now, recounting the exact moment they made the decision to be the woman they are now and are happy and proud of themselves for it, to seeing whoever they were before as a stranger, someone depressed and suicidal before she finally found purpose and pride after "the change". Others don't bother remembering their past, the person they used to be simply not mattering because who they are now, the name they use now, that is what matters.
The main character of the story is a man who sees what is happening to these women and is told by the status quo that he is not only encouraged but expected to participate in this systemic violence, and is so horrified by the experience that he devotes his entire being to fighting for their rights and trying to support them, to the point that he's willing to dehumanize and sacrifice himself if it means even one of them can be happy. Despite being described multiple times as nigh superhuman and the ideal man, he is functionally powerless and his primary role is to provide emotional support, friendship, and advocacy for the women that actually move the plot and make things happen, because the only way society will take them seriously is if a man vouches for them. One of his and his allies' main goals is to overturn the government and reform society so that these women will not be subject to this objectification and discrimination, and the main method through which this is accomplished is by showing solidarity with those the government deems undesirable, refusing to engage in the systemic discrimination the government enforces on society. Also he gets force femmed, instant lossed, pegged, beat up for being a "robot fucker", dom'd by lesbians, roofied, and much more.
And then we go into the individual character stories, some of which are very bluntly and transparently allegories for real life issues faced by transwomen. Things like parental abandonment after coming out, autism and dysphoria resulting in crippling depression, passing in a transphobic workplace, struggling to have your accomplishments as an athlete recognized, just to name a few. The ShiftUp writing team either has transwomen in it, or have a lot of transfem friends.
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