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qPCR and extraction of DNA
This morning I had the opportunity to practice DNA extraction from a blood sample from a South hospital. It was a rather nice experience since I wasn't anxious at all, I had overcome my fear of micropipette.
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Days until thesis submission: 102
I’m struggling with motivation and mental health leading up to my thesis submission so I thought I’d make a countdown of sorts! Every day I’m going to post my productivity and something that made me smile to help keep me going! My submission date is August 31st so it works pretty well with the 100 days of productivity challenge too, feel free to join :)
Microplaca para uso em termociclador para análises de qPCR. https://www.produtosparalaboratorios.com.br/microplaca-qpcr-96-pocos Compatibilidade K7-9612-W: Marca Termocicladores ABI: 9600, 9800 fast, StepOne Plus Biometra: T1, Trobot BioRad:Ptc-100, Ptc-200, Ptc-225, Opticon, Opticon2, Chromo4, C-1000, S-1000, CFX96 Eppendorf: MasterCycler, MC Gradient Esco: Swift Maxi Euroclone: Onde, PeqSTAR Roche: LightCycler 480, Light Typer Thermo Hybaid: MultiBlock System Marca Termocicladores qPCR ABI: StepOne Plus BioRad: Opticon, Opticon2, Chromo4, CFX96 Roche: LightCycler 480 @teratecsp #teratecsp #apoieaspequenasempresas #compredopequeno #compromisso #comprometimento #kasvi #produtosparalaboratorios #analisesclinicas #pcr #qpcr #termocicladores [email protected] https://wa.me/5511941431076 (em Teratec Soluções em Precisão Ltda) https://www.instagram.com/p/CC_qicRAKob/?igshid=w01gx7ifhpms
10/31/19 Daaaaamn! Look at this qRT-PCR! Now THIS is sexy! This should be my Sexy Halloween Costume!
African Swine Fever (ASF) is a highly lethal transboundary disease that endangers swine production and food security. This study conducted active molecular surveillance to detect ASF virus (ASFV) in raw pork and whole-blood samples collected from local government–sanctioned wet markets in Tuguegarao City. From August to October 2025, a total of 180 specimens (30 meat and 30 whole-blood samples per month) were collected, pooled by stall owner and market, and transported cold for laboratory processing. Nucleic acids were extracted and screened using real-time PCR assays targeting the VP72 (B646L) gene, with appropriate extraction, weak and strong positive, and no-template controls to ensure run validity. Results showed that all specimens produced undetermined cycle threshold (Ct) values and lacked characteristic amplification curves, yielding 0/180 PCR positives. Quality control amplification of positive controls and flat negative controls confirmed assay performance. Exact Clopper–Pearson binomial analysis generated a two-sided 95% confidence interval of 0.00 to 0.02029 for true prevalence, placing an upper bound of 2.03%. These findings indicate ASFV was not detected from any of the samples analyzed, while acknowledging potential limitations such as pooling, sample size, viral loads below the assay limit of detection, or nucleic acid degradation. The study recommends sustained surveillance with increased sample numbers and duration, biosecurity assessments at market and farm levels, and data linkage with live-swine surveillance to strengthen early detection and protect local pork value chains.
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التكنولوجيا الحيوية في خدمة سلامة الغذاء.. تجربة طلابية تكشف السالمونيلا بدقة عالية
Waste Water Testing | Pathogen Detection & Monitoring
Eurofins Genomics offers waste water testing using advanced qPCR and sequencing to detect pathogens, SARS-CoV-2, and antimicrobial resistance genes.
Digital PCR & qPCR Market
Digital PCR (dPCR) and quantitative PCR (qPCR) markets are rapidly expanding, driven by the growing demand for precise genetic analysis, diagnostics, and research applications. dPCR offers highly accurate quantification of nucleic acids, while qPCR is widely used for gene expression profiling and pathogen detection. Both technologies are advancing in fields such as oncology, infectious disease monitoring, and genetic research. The market growth is fueled by technological innovations, increased adoption in clinical diagnostics, and a rising focus on personalized medicine. Key players in the market include Thermo Fisher Scientific, Bio-Rad, and QIAGEN, with significant investment in R&D and product development.
For More : https://tinyurl.com/bdhjhwkf
