Well what do we have here?

[Q: Why did you decide to do this experiment?

A: Snake behavior has been generally neglected as a field of research, especially in Brazil. And most studies don’t examine what factors make them want to bite. If you study malaria, you can research the parasite that causes the disease—but if you don’t study the mosquito that carries it, you will never solve the problem. Up until now, the popular wisdom was that the jararaca would only attack if you touched it or stepped on it. But that was not what we found.

Q: Why did you need to be the victim?

A: The best way to do this research is to put snakes and a human together. In this case, the human was me. We put the snakes inside a ring on the floor of our lab until they got used to it, then I stepped in wearing special protective boots. I stepped close to the snake and also lightly on top of it. I didn’t put my whole weight on my foot, so I did not hurt the snakes. I tested 116 animals and stepped 30 times on every animal, totaling 40,480 steps.]

The backgrounds for the entire series were done on black paper, a first for any animated show. It's Batman, which means everything is at night and incredibly dark. So, the majority of the backgrounds were going to be black anyways and using black paper would cut down on the time it took to create all of the backgrounds. Adding color over the black paper instead of the traditional method gave it a much more stylized look that helped make it iconic.

It also forced some other creative choices, such as using an airbrush to do all of the background art. During the early testing, they found that using an airbrush was easier to apply paint with than a paintbrush and that it looked better. Airbrushing let them be more experimental with painting techniques, such as the spattery fades you can see in the "Bane" and "Deep Freeze" title cards above or the soft, deeply shadowed face on "the Last Laugh." The overall effect of airbrushing on a black background is a much darker, moodier vibe than could be achieved with a traditional approach.

https://www.goodreads.com/book/show/7981801-putting-food-by