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Multiphoton fluorescence image of HeLa cells with cytoskeletal microtubules (magenta) and DNA (cyan). Nikon RTS2000MP custom laser scanning microscope.
Source: National Center for Microscopy and Imaging Research (NCMIR) (source)
Credit Line: National Center for Microscopy and Imaging Research
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Collective invasion of B16/F10 melanoma cells into mouse dermis, detected by infrared multiphoton microscopy. Tumor cells expressing E2-Crimson and Histone-2B-GFP are (false-colored) yellow (cytoplasm) and blue (nuclei). Nerve fibers and fat cells are cyan (third harmonic) and collagen bundles and muscle fibers are red (second harmonic), respectively. Blood vessels and phagocytic cells are labeled with TM-Rhodamine-dextran in green. By courtesy of Bettina Weigelin and Peter Friedl, NCMLS, Radboud University, Nijimegen, Netherlands.
(Source: https://www.lavisionbiotec.com/applications/oncology.html)
Alexander, S., Weigelin, B., Winkler, F., Friedl, P., Preclinical intravital microscopy of the tumour-stroma interface: invasion, metastasis, and therapy response, Curr Opin Cell Biol. (2013), 25(5), 659-71
Scientists combined two different high-tech optical imaging methods (multiphoton laser-scanning microscopy (MPLSM) and optical frequency domain imaging (OFDI)) in order to track the movement of molecules, cells and fluids within tumors and examine abnormalities in blood vessel networks. The method can be used to study how different treatments affect cancer tumours and promises to help researchers better understand the intricate workings of human cancer. Credit : Dai Fukumura et al.
Multiphoton microscopy of mouse motor neurons by ZEISS Microscopy on Flickr.
Mouse Large Intestine Technique : Multiphoton
Dr. Paul Appleton Blairgowrie, UK
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A multiphoton image of a human adenoma, a benign tumor. Image by Aaron Quyn, University of Dundee.
