Collective invasion of B16/F10 melanoma cells into mouse dermis, detected by infrared multiphoton microscopy. Tumor cells expressing E2-Crimson and Histone-2B-GFP are (false-colored) yellow (cytoplasm) and blue (nuclei). Nerve fibers and fat cells are cyan (third harmonic) and collagen bundles and muscle fibers are red (second harmonic), respectively. Blood vessels and phagocytic cells are labeled with TM-Rhodamine-dextran in green. By courtesy of Bettina Weigelin and Peter Friedl, NCMLS, Radboud University, Nijimegen, Netherlands.
(Source: https://www.lavisionbiotec.com/applications/oncology.html)
Alexander, S., Weigelin, B., Winkler, F., Friedl, P., Preclinical intravital microscopy of the tumour-stroma interface: invasion, metastasis, and therapy response, Curr Opin Cell Biol. (2013), 25(5), 659-71
