LC Sciences

Sequencing Platform

Illumina NovaSeq X Plus high-throughput sequencing platform for large-scale genomic projects

NovaSeq X Plus:

As one of the two latest Illumina sequencing systems, the NovaSeq X Plus allows us to offer maximum throughput and accuracy, as well as exceptional sequencing capabilities for data-intensive applications.

10 B (PE150) Flow Cell available:   ~1.25 B paired reads per lane.

25 B (PE150) Flow Cell available:   ~3.2 B paired reads per lane.

NovaSeq 6000:

With the NovaSeq 6000, we are able to sequence up to 64 human genomes with 90 Gb and produce 6 Tb of data per single run as short as 40 hours, providing users with the speed and flexibility to complete projects faster and with greater output.

50 G raw data per lane, 3.4 T raw data per Flowcell, and 2 × 150 bp read length.

800 M raw reads per flow cell, with 2 × 250 bp and 2 × 50 bp read lengths.

Single Cell Platform:

The Chromium X makes single-cell analysis easy, reliable, and scalable thanks to its proprietary Next GEM technology. With Chromium X, we can conduct any 10x Genomics single-cell experiment on the scale you need, including single-cell transcriptomes, single-cell immunobanks, single-cell ATACs, and multi-omics consortia.

Automated intelligent NGS library preparation workstation in LC Sciences genomics laboratory

Intelligent NGS Workstation:

LC Sciences provides industry-leading speed from sample to data, powered by its fully automated, round-the-clock NGS solutions. Introducing the UnityFX series—an all-in-one automation platform for NGS library construction and pooling. Engineered for true walkaway operation, UnityFX enables continuous, unmanned processing, dramatically increasing workflow efficiency and accelerating project turn-around time for clients. This highly integrated system sets a new benchmark in robustness and reproducibility, helping to push the boundaries of genomic science and foster a more scalable NGS ecosystem.

Computing Platform

We have constructed a substantial data processing facility that encompasses an area of over 150 square meters. This facility, comprises more than 300 computing nodes and features a distributed storage capacity of over 50 petabytes.

LC Sciences provides a DNA/RNA Aptamer Binding service using microarrays based on our in-house developed µParaflo® technology platform.

Single-cell gene expression analysis is a technique that enables the study of gene expression at the individual cell level, offering higher resolution and greater insight into cellular heterogeneity compared to traditional bulk RNA-Seq. Single-cell RNA sequencing (scRNA-Seq) provides a deeper understanding cellular function within the context of the surrounding microenvironment.

Applications

Construction of large-scale cell atlases

Tracing the development and differentiation of stem cells

Investigation of tumor initiation, maintenance, and evolution

Single Cell RNA Sequencing (single-cell RNA-seq or scRNA-seq) Advantages

Advanced technology and instruments

In collaboration with 10x Genomics, we have established a joint laboratory for single-cell sequencing. This initiative enables the rapid and accurate sorting and validation of target cell populations, ensuring high-quality data generation.

Extensive experience

Our laboratory has developed and optimized sample preparation protocols for over 350 tissue types across more than 50 species. To date, we have completed over 10,000 single-cell preparations and scRNA-Seq experiments.

Professional team

Our experienced experts provide comprehensive support in experimental design, data analysis, and result interpretation.

Reliable and fast turnaround time

A dedicated project manager ensures that results are delivered within 30 business days of sample quality verification.

Our Comprehensive Service

At LC Sciences, we offer a genome-wide microRNA expression profiling service utilizing a microarray detection system that was developed specifically for microRNA detection.  Send us your total RNA sample and we’ll perform all the necessary functions from sample QC through data analysis.  We can perform microarray analysis on a single sample to create a simple expression profile or we can hybridize two samples to the same microarray for “dual sample” analysis.  This is very useful whenever comparison of two samples is needed such as wildtype vs. mutant or samples treated in two different ways.

Our “Total RNA to Data” comprehensive service includes: Sample QC, Sample Preparation and Labeling, microRNA Detection (hybridization to a µParaflo® microfluidics chip), Array Scan and Data Extraction, and Full Data Analysis.

In about 2-3 weeks you will receive: original and processed scan images, array layout and a list of sequences,  raw and processed data, a list of differentially expressed transcripts (dual sample arrays only), and a summary of the results.

LC Sciences provides a microRNA (miRNA) expression profiling service using microarrays based on our in-house-developed μParaflo® technology platform.

In recent years, RNA modifications have attracted increasing scientific interest, particularly N6-methyladenosine (m6A)—the most prevalent methylation modification observed in mRNA. Methylated RNA immunoprecipitation sequencing (MeRIP-seq or m6A-seq) is a highly effective method for detecting m6A and other post-transcriptional RNA modifications. Furthermore, this method enables genome-wide identification of m6A sites, allowing researchers to quantify modification levels across genes and perform comparative analyses between groups.

Applications

Investigation of mRNA translation and degradation

Study of embryonic development and cellular differentiation

Analysis of disease pathogenesis mechanisms

m6A RNA Sequencing (m6A-seq) Advantages

Strategic collaboration

We have partnered with a leading RNA methylation team to streamline the experimental and analytical processes.

Extensive experience

We have constructed over 10,000 m6A-Seq libraries and supported our clients in publishing more than 200 scientific papers, over half of which appeared in high-impact journals (IF > 10).

Specialist team

Our team includes experienced professionals who can assist you with experimental design, data analysis, and result interpretation.

Reliable and fast turnaround time

A dedicated project manager ensures that results are delivered within 20 business days of sample quality verification.

Research team

Ensuring the highest level of data quality and reproducibility.

Our Comprehensive Service

At LC Sciences, we offer a genome-wide microRNA expression profiling service utilizing a microarray detection system that was developed specifically for microRNA detection.  Send us your total RNA sample and we’ll perform all the necessary functions from sample QC through data analysis.  We can perform microarray analysis on a single sample to create a simple expression profile or we can hybridize two samples to the same microarray for “dual sample” analysis.  This is very useful whenever comparison of two samples is needed such as wildtype vs. mutant or samples treated in two different ways.

Our “Total RNA to Data” comprehensive service includes: Sample QC, Sample Preparation and Labeling, microRNA Detection (hybridization to a µParaflo® microfluidics chip), Array Scan and Data Extraction, and Full Data Analysis.

In about 2-3 weeks you will receive: original and processed scan images, array layout and a list of sequences, raw and processed data, a list of differentially expressed transcripts (dual sample arrays only), and a summary of the results.

LC Sciences offers microRNA (miRNA) expression profiling services using microarrays built on our in-house-developed μParaflo® technology platform.

MicroRNAs (miRNAs) are small, non-coding RNA molecules that act as negative regulators of gene expression, controlling a wide range of cellular processes. Research on microRNAs is rapidly expanding as researchers identify new microRNAs and uncover their critical roles in diverse biological systems.

MicroRNA Sequencing (miRNA-seq) Advantages

Unique analysis software

We are pleased to announce the introduce ACGT101-miR, our new data analysis software. This innovative tool generates publication-ready charts, providing a streamlined and efficient way to visualize and present your findings.

Extensive experience

As a pioneer in microRNA-seq services, our clients have published over 980 microRNA studies, including more than 200 high-impact publications (IF > 10).

Specialist team

Our team includes experienced professionals who can assist you with experimental design and data interpretation.

Reliable and fast turnaround time

A dedicated project manager ensures that results are delivered within 20 business days of sample quality verification.

Our Comprehensive Service

At LC Sciences, we offer a genome-wide microRNA expression profiling service utilizing a microarray detection system that was developed specifically for microRNA detection.  Send us your total RNA sample and we’ll perform all the necessary functions from sample QC through data analysis.  We can perform microarray analysis on a single sample to create a simple expression profile or we can hybridize two samples to the same microarray for “dual sample” analysis.  This is very useful whenever comparison of two samples is needed such as wildtype vs. mutant or samples treated in two different ways.

Our “Total RNA to Data” comprehensive service includes: Sample QC, Sample Preparation and Labeling, microRNA Detection (hybridization to a µParaflo® microfluidics chip), Array Scan and Data Extraction, and Full Data Analysis.

In about 2-3 weeks you will receive: original and processed scan images, array layout and a list of sequences, raw and processed data, a list of differentially expressed transcripts (dual sample arrays only), and a summary of the results.

LC Sciences provides specialized epigenomics services, including Whole Genome Bisulfite Sequencing (comprehensive single-base resolution DNA methylation analysis), Reduced Representation Bisulfite Sequencing (targeted CpG-rich region study with lower cost), ATAC-seq (chromatin accessibility and regulatory element investigation), and CUT&Tag (high-resolution chromatin profiling for small/single-cell samples).

Whole Genome Bisulfite Sequencing offers single-base resolution for thorough methylation insights; Reduced Representation Bisulfite Sequencing balances efficiency and cost with targeted CpG-rich region focus; ATAC-seq enables efficient detection of open chromatin and regulatory elements; CUT&Tag excels in high-resolution profiling even with small or single-cell samples.

Whole Genome Bisulfite Sequencing

Whole Genome Bisulfite Sequencing (WGBS) is a bisulfite sequencing method that enables the detection of DNA methylation at the whole-genome level. This encompasses the analysis of methylation at CpG sites and less common non-CpG sites, including CNG. The detection and quantification of methylation are of critical importance for the understanding of gene expression and other processes that are subjected to epigenetic regulation.

Reduced Representation Bisulfite Sequencing

Reduced representation bisulfite sequencing (RRBS) is an efficient and high-throughput technique for analysing the genome-wide methylation profiles on a single nucleotide level. It combines restriction enzymes and bisulfite sequencing to enrich for areas of the genome with a high CpG content. Given its cost-effective and productive nature, RRBS is employed extensively across a range of fields.

Assay for Transposase-Accessible Chromatin Sequencing (ATAC-seq)

ATAC-seq (Assay for Transposase Accessible Chromatin using sequencing) is a high-throughput sequencing technology that uses the transposase Tn5 to investigate chromatin accessibility. The core principle of ATAC-seq is to use the transposase Tn5 to cleave open chromatin regions and, in doing so, to incorporate sequencing adapters into the DNA at these regions. These adapter-tagged DNA fragments are then subjected to high-throughput sequencing to identify open chromatin regions in the cell nucleus. These regions are typically sites in the genome that are readily accessible to transcription factors and other regulatory proteins. Therefore, ATAC-seq is a powerful tool for studying epigenetic changes in gene expression regulation, cell differentiation, and disease

Cleavage Under Targets and Tagmentation (CUT&Tag)

CUT&Tag (Cleavage Under Targets and Tagmentation) is an advanced epigenetics technology that enables high-sensitivity detection of chromatin protein binding sites with low input or even single-cell samples. Compared with traditional ChIP-seq, CUT&Tag offers a simpler workflow, lower background noise, and higher resolution, providing more precise insights into regulatory mechanisms.

Sequencing Platform

Our services are powered by the Illumina, enabling us to provide high-quality data that fully meets your scientific research needs.

RNA Sequencing

LC Sciences offers a range of rapid and comprehensive RNA sequencing solutions, including poly-A RNA, micro RNA, total RNA, circRNA, m6A RNA, and more.

LC Sciences provides a fast, comprehensive microbial Sequencing solution including: 16S/18S/ITS Amplicon Sequencing, 5R 16S Amplicon Metagenomic Sequencing, Shotgun Metagenomic Sequencing.

Microbial sequencing is a technology that employs a range of high-throughput genomics techniques to examine microbial communities and their functions. This encompasses the utilisation of amplicon sequencing techniques to examine the composition and configuration of microbial communities. LC Bio offers a rapid and comprehensive microbial sequencing solution, which includes: 16S/18S/ITS amplicon metagenomic sequencing, 5R 16S amplicon metagenomic sequencing, shotgun metagenomic sequencing.

16S/18S/ITS Amplicon Sequencing

16S/18S/ITS amplicon sequencing is a widely used method for the identification and differentiation of microbial species. Short hypervariable regions of conserved genes or intergenic regions—such as the 16S region of bacteria and archaea or the 18S/ITS region of fungi—are amplified by PCR and subsequently analyzed using next-generation sequencing (NGS) technology. The resulting data can be applied to numerous areas within the field of microbial research.

5R 16S Amplicon Metagenomic Sequencing

5R 16S sequencing is a microbiota assay that utilizes multiplexed PCR amplification and sequencing of distinct five regions of the 16S rRNA gene minimizes the significant interference of host DNA with bacterial 16S rDNA amplification in tissue samples. By integrating a dedicated contaminant filtration process, which enables researchers to comprehensive and accurate profiling microbiota characteristics and functions in tissues with low microbial biomass and a high proportion of host DNA.

Shotgun Metagenomic Sequencing

Metagenomics is the study of genetic material obtained directly from complex environmental or human samples (such as soil, water, or gastrointestinal tract samples). Metagenomic sequencing provides insights not only into species identification, classification, and quantification of microbes, but also into functional genes, inter-sample gene variation and metabolic pathways.

Sequencing Platform

Our services are powered by the Illumina, enabling us to provide high-quality data that fully meets your scientific research needs.

DNA Sequencing

LC Sciences provides a variety of fast, comprehensive DNA sequencing solutions, including exome, whole-genome, whole-genome bisulfite, and reduced representation bisulfite sequencing.

The advent of high-throughput sequencing technology has facilitated a deeper comprehension of the interrelationship between genomic variation/gene expression differences and phenotype. However, traditional bulk RNA sequencing methods are targeted at a collection of cells, and the resulting data often fails to capture the full range of information present at the single-cell level, leading to the loss of significant insights. The microfluidic technology of the 10x Genomics platform enables the concurrent acquisition of gene expression data, TCR/BCR sequence information and chromatin openness information from a large number of single cells. This is achieved by sorting single cells, thus facilitating rapid and efficient labelling, sequencing and analysis of a large number of cells. The gene expression profiles and differences at the single-cell level are obtained, and large-scale single-cell expression maps are generated through in-depth and detailed analysis of complex cell populations. The project has a relatively short project cycle and a wide range of potential applications. It is capable of producing large-scale single-cell expression profiles.

Single Cell RNA Sequencing (single-cell RNA-seq or scRNA-seq)

Single-cell gene expression analysis is a technique that enables the study of gene expression at the individual cell level, offering higher resolution and greater insight into cellular heterogeneity compared to traditional bulk RNA-Seq. Single-cell RNA sequencing (scRNA-Seq) provides a deeper understanding cellular function within the context of the surrounding microenvironment.

Single Cell Immune Profiling (scVDJ-seq)

Single-cell immune enables the comprehensive classification of immune cells across hundreds to tens of thousands of distinct populations. By analyzing cell surface markers, cytokine expression, and protein phosphorylation states researchers can precisely characterize immune subpopulations within heterogeneous cell mixtures.

Single Cell ATAC Sequencing (scATAC-seq)

Single-cell ATAC sequencing (scATAC-Seq) enables researchers to capture the chromatin accessibility profiles of tissues at single-cell resolution. By analyzing these profiles, scientists can uncover how tissues and individual cell types change during disease progression or respond to external stimuli and compounds through alterations in chromatin accessibility.

Sequencing Platform

The 10x Genimics platform enables us to provide high-quality data that is fully aligned with the requirements of scientific research.

LC Sciences offers a range of rapid and comprehensive RNA sequencing solutions, including poly-A RNA, micro RNA, total RNA, circRNA, m6A RNA, and more.

Our comprehensive sequencing services utilizes the latest next-generation sequencing technologies. Illumina’s industry-leading RNA sequencing methods facilitate the discovery and profiling of RNAs in any organism without the need for prior genome annotation. They also enable the most accurate detection and quantification of rare RNA sequences and sequence variants in your samples.

Poly (A) Sequencing (mRNA-seq)

Poly (A) RNA-Seq is an advanced method for accurately measuring gene expression in biological samples under specific conditions. It enables the identification of novel transcripts, alternative splicing, and gene fusion events, providing a comprehensive view of the transcriptome.

MicroRNA Sequencing (miRNA-seq)

MicroRNAs (miRNAs) are small, non-coding RNA molecules that act as negative regulators of gene expression, controlling a wide range of cellular processes. Research on microRNAs is rapidly expanding as researchers identify new microRNAs and uncover their critical roles in diverse biological systems.

Total RNA Sequencing

Long non-coding RNAs (lncRNAs) are RNA molecules longer than 200 nucleotides that do not encode proteins. The involvement of lncRNAs in a wide range of essential cellular processes, coupled with their critical roles in human diseases such as cancer, cardiovascular disorders, and neurological conditions, makes them a significant focus of current research. lncRNA-Seq is a valuable tool for obtaining a comprehensive view of lncRNA expression in biological samples under specific conditions.

Circular RNA Sequencing (circRNA-seq)

Circular RNAs (circRNAs) represent a novel class of abundant, stable, and ubiquitous RNA molecules. Unlike mRNAs, circRNAs lack poly (A) tails and 5’ caps, and are resistant to exonuclease degradation. circRNAs can act as microRNA (miRNA) or RNA-binding protein (RBP) sponges, sequestering miRNAs and sequestering these molecules and thereby regulating gene expression and transcriptional activity.

m6A RNA Sequencing (m6A-seq)

In recent years, RNA modifications have attracted increasing scientific interest, particularly N6-methyladenosine (m6A)—the most prevalent methylation modification observed in mRNA. Methylated RNA immunoprecipitation sequencing (MeRIP-seq or m6A-seq) is a highly effective method for detecting m6A and other post-transcriptional RNA modifications. Furthermore, this method enables genome-wide identification of m6A sites, allowing researchers to quantify modification levels across genes and perform comparative analyses between groups.

Whole Transcriptome Sequencing (WTS)

Whole Transcriptome Sequencing (WTS)—which combines RNA-Seq with rRNA removal and small RNA-Seq—is a powerful tool that provides a comprehensive overview of mRNAs, lncRNAs, circRNAs, and microRNAs, in biological samples under specific conditions. WTS enables the investigation of complex post-transcriptional regulatory mechanisms, particularly the competitive endogenous RNA (ceRNA) network. In this network, ceRNAs such as mRNAs, lncRNAs, and circRNAs act as molecular sponges for microRNAs (miRNAs) by binding to their miRNA recognition sites, thereby relieving repression on the target genes of the corresponding miRNA family.

Low-Input RNA Sequencing

Low-Input RNA Sequencing （Smart Pico RNA Sequencing）, powered by the SMARTer Stranded Total RNA-Seq Kit v2 - Pico Input Mammalian, is an ultra-sensitive RNA-seq solution specifically designed for challenging samples such as formalin-fixed paraffin-embedded (FFPE) tissues and extremely limited inputs as low as 10 pg. This technology overcomes the limitations of conventional methods regarding sample integrity and input requirements.

Sequencing Platform

Our services are powered by the Illumina, enabling us to provide high-quality data that fully meets your scientific research needs.

LC Sciences provides a variety of fast, comprehensive DNA sequencing solutions, including exome, whole-genome, whole-genome bisulfite, and reduced representation bisulfite sequencing.

Our next-generation DNA sequencing services utilise the latest in deep sequencing technologies. The VariantPro™ targeted sequencing system, when used in conjunction with Illumina’s industry-leading DNA sequencing methods, enables the discovery of germline or somatic mutations and the detection of low-frequency alleles.

Human Whole Genome Sequencing

Whole-genome sequencing (WGS) provides the most comprehensive insight into the genetic code. It enables the identification of genomic variations, including single-nucleotide polymorphisms (SNPs), insertions and deletions (indels), structural variations (SVs), and copy number variations (CNVs), all within a single, cost-effective assay.

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Animal and Plant Whole Genome Sequencing

Whole-genome sequencing of animals and plants provides a comprehensive understanding of the DNA sequences within their genomes. The resulting marker loci can be used to analyze data at both the individual and population levels, facilitating the study of other genomic variants. This represents a crucial step toward improving plant and animal species and advancing agricultural development.

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Human Whole Exome Sequencing

The human exome is estimated to contain approximately 180,000 exons, which constitute about 1% of the human genome, or roughly 30 megabases. The objective of this approach is to identify functional variations responsible for both Mendelian and common diseases while accounting for the high costs associated with whole-genome sequencing and the need to maintain high sequencing depth. Therefore, whole-exome sequencing (WES) represents a less costly yet still highly effective alternative to whole-genome sequencing (WGS).