These are two canonically trans characters. So, lets look at this interaction from that perspective.
Huntress sees Hunter and without knowing anything more about him she assumes he's weak. Because she sees a version of herself, a trans woman, that is presenting as a man. So, she assumes Hunter is a version of herself that hasn't transitioned, that is *too weak* to transition. Which also drives home in her mind that she is *the* version of Huntress Wizard and Hunter is just a weak copy of herself.
But Hunter just sees a version of himself, nothing else exceot that this version of himself is rude and putting on a "tough guy" personna. Possibly he also sees a pre-transition version of himself. Maybe before transition Hunter was more closed off and abrasive. I know I was when I was living in the closet. So it doesn't even occur to him that calling Huntress "guy" would be a problem.
Which gets Hunter rightfully headbutted.
(It happened so fast I had to pause the clip to get a screenshot and so I couldn't get it without all the youtube bullshit in the shot, lol)
I love this whole interaction so fucking much. It's pretty much *exactly* how I expected it would go. Like, long before this episode aired I imagined how I would react if I encountered a version of me that was a transmasc, and that would probably be what I would think upon seeing them as well; a version of me that never found the strength to transition.