#issr

Olea europaea is an economically important tree species native to Mediterranean region. The present study is focused on developing the regeneration protocol and production of true-to-type plants of Olea europaea L. cv Barnea with commercial importance. Olive tree is a long lived evergreen tree but has a long juvenile phase and reaches its full production stage at 25 - 30 years of age. Traditionally, for many years, they have been propagated through cutting, grafting, vegetative propagation and in recent years, through air layering through plant tissue culture methods this problem of long juvenility can be overcome in addition large number of pathogen free plants can be produced. Nodes from adventitious shoots were surface sterilized and inoculated in different tissue culture media such as Woody Plant Media (WPM), Murashige and Skoog media (MS), Rugini Olive media (OM), Schenk and Hildebrandt media (SH) and Multiplication media (Combination of half strength MS and half strength Rugini OM) in which culture establishment was best obtained in multiplication media supplemented with 1 mg/l zeatin. Different treatment was given to induce shoot proliferation and the highest shoot number 4.2±0.4 was obtained in multiplication media enriched with 2.0 mg/l zeatin and silver nitrate at 2.0 mg/l. The in-vitro derived shoots were assessed for genetic fidelity using different molecular markers Random Amplification of Polymorphic DNA (RAPD) and Inter Simple Sequence Repeat (ISSR). Monomorphic bands were obtained between the mother DNA and in-vitro derived progenies which indicated that there is genetic stability in the in-vitro regenerated plants. Commercial analysis was done with respect to Industrial Use. The global market for olive is estimated to grow at a compound annual growth rate of 4.5% in the forecast period of 2019-2024. In the production of olive, Spain holds the largest share of world production and Italy holds the second largest share. Although in recent years, the world has begun to see olive from various new regions. The present study is the initiative step towards propagation of Olive cultivar in South Asian region.

Author(s) Details

Dr. (Mrs.) Susmita Shukla Applied Plant Biotechnology Research Laboratory, Centre for Plant and Environmental Biotech, Amity Institute of Biotechnology, Amity University, Noida (U.P), India.

Dr. Shiv Kant Shukla Biotech Consortium India Limited (BCIL), Anuvrat Bhawan, 210 DDU Marg, New Delhi 02, India.

Taramla Raman Applied Plant Biotechnology Research Laboratory, Centre for Plant and Environmental Biotech, Amity Institute of Biotechnology, Amity University, Noida (U.P), India.

The species Syagrus oleracea is a native palm tree, present in highly impacted environments of the Brazilian semiarid. This species is exploited for use in the manufacture of folk crafts and for human and animal feeding. The objective of this study was to characterize the diversity and genetic structure of individuals from different populations, located in anthropic regions of the Brazilian semiarid region, in the state of Minas Gerais. This information is important for species management and conservation strategies. Thus, we used ISSR molecular markers in 157 individuals in seven populations of S. oleracea. Looking at the results, one can observe that amplifications of 109 locos occurred and that an average of 18, 17 alleles were found on each primer, values that demonstrated that the ISSR technique used was highly efficient. The genetic diversity indexes were observed, showing expected heterozygosity (He) and Shannon index (I) of 0.260 and 0.383, respectively. The number of alleles observed (na) among populations was 1.954 and the effective number of alleles (ne) was 1,606. Individuals of the populations are genetically structured in groups (demes) in a random and aggregate manner. We obtained the amplification of 109 ISSR loci, with a mean of 18.2 alleles per primer. The genetic diversity indexes revealed expected heterozygosity (He) and Shannon index (I) of 0.260 and 0.383, respectively. The number of alleles observed (na) was 1.954 and the effective number of alleles (ne) was 1.606. The value of the indirect gene flow found was 1.546. Evidence of high levels of kinship in populations was found, possibly due to low migration rates and geographical barrier. In addition, we observed signs of overexploitation in the areas, which consequently leads to a low rate of gene flow, occurring inbreeding among the sampled populations of Syagrus oleracea.

Author(s) Details

Luciana Rodrigues de Souza Laboratory Bioprospecting and Genetic Resources and Laboratory of Natural Products, State University of Montes Claros, Campus Darcy Ribeiro, Vila Mauriceia, Montes Claros, MG, Brazil.

Helbert Fagundes Soares Laboratory Bioprospecting and Genetic Resources and Laboratory of Natural Products, State University of Montes Claros, Campus Darcy Ribeiro, Vila Mauriceia, Montes Claros, MG, Brazil.

Sara Luiza Ramos Ribeiro Laboratory Bioprospecting and Genetic Resources and Laboratory of Natural Products, State University of Montes Claros, Campus Darcy Ribeiro, Vila Mauriceia, Montes Claros, MG, Brazil.

Afranio Farias de Melo Junior Laboratory Bioprospecting and Genetic Resources and Laboratory of Natural Products, State University of Montes Claros, Campus Darcy Ribeiro, Vila Mauriceia, Montes Claros, MG, Brazil.

Murilo Malveira Brandao Laboratory Bioprospecting and Genetic Resources and Laboratory of Natural Products, State University of Montes Claros, Campus Darcy Ribeiro, Vila Mauriceia, Montes Claros, MG, Brazil.

Elytania Veiga Menezes Laboratory Bioprospecting and Genetic Resources and Laboratory of Natural Products, State University of Montes Claros, Campus Darcy Ribeiro, Vila Mauriceia, Montes Claros, MG, Brazil.

Vanessa de Andrade Royo Laboratory Bioprospecting and Genetic Resources and Laboratory of Natural Products, State University of Montes Claros, Campus Darcy Ribeiro, Vila Mauriceia, Montes Claros, MG, Brazil.

Dario Alves de Oliveira Laboratory Bioprospecting and Genetic Resources and Laboratory of Natural Products, State University of Montes Claros, Campus Darcy Ribeiro, Vila Mauriceia, Montes Claros, MG, Brazil.

Genetic variability and phylogenetic relationships studies of Aegilops L. using some molecular markers – IJAAR

Mahjoub Asma, Mguis Khaled, Ben Brahim Nadia

Laboratory of botanic of INRAT, Hedi Karry Street, Ariana, Tunisia

Department of Biology, Faculty of Sciences of Tunis, University of Tunis El Manar, Tunis, Tunisia

Key words: Aegilops L., AFLP, ISSR, IRAP, Molecular markers, RAPD, SSR.

Abstract

Studying of genetic relationships among Aegilops L. species is very important for broadening the cultivated…