Here, imaging-based CRISPR screening of the genome (to mutate genes of interest), allows cell-regulating molecules and modifications to be revealed in live and fixed cells. Primary cilia – whiskery functional projections found on most cell types – are studied here as a proof of concept and several previously unknown ciliary regulators identified and characterised, such as microprotein TZMP1. Shown are tadpole skin cells with cilia – left, unmanipulated and right, after CRISPR-targeting the gene encoding TZMP1
Read the published research article here
Video from work by Jingbo Sun and colleagues
Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, CT, USA
Video contributed by the authors and originally published with a Creative Commons Attribution 4.0 International (CC BY 4.0)
Published in Developmental Cell, November 2025
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