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Selecting analytes for System Suitability Testing (SST) in GC/HPLC sounds simple… until you actually have to do it.

It’s one of those steps that quietly decides whether your method is truly reliable—or just “passing” on paper.

The truth? The analyte you choose defines how well your system performance is being tested.

Here’s what a good SST analyte looks like: • Easily available • Stable • Cost-effective • Strong detector response • Good solubility • Relevant to your method • Appropriate polarity

Small decision, big impact.

Struggling with poor peak separation? Here are quick, practical tips to boost your HPLC resolution:

1. Increase Column Efficiency (N) Use smaller particle size columns, longer columns, and avoid overloading your sample.

2. Improve Selectivity Switch stationary phase (C18 → phenyl/cyano) or adjust mobile phase (pH, solvents like ACN/MeOH).

3. Optimize Retention (RT) Tweak mobile phase strength, flow rate, or use gradient elution.

4. Reduce Particle Size Smaller particles (e.g., 3.5 µm vs 5 µm) = better separation.

5. Smart Lab Practices Use guard columns, proper flushing, and temperature control for consistent results.

Small changes = sharper peaks + better data!

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An SOP (Standard Operating Procedure) outlines general instructions for routine tasks, ensuring consistency and efficiency. In contrast, STP refers Standard Testing Procedure related to the method of analysis. While the SOP provides the overall framework for operations, the STP details the precise steps for conducting a specific test, ensuring accuracy

👉 Read the Full Article on SOP Vs STP

Related substances and assay tests are critical quality control measures in pharmaceuticals. The related substances test detects and quantifies impurities, while the assay test measures the active pharmaceutical ingredient (API) content. Together, they ensure the drug's purity, potency, safety, and overall quality.

👉 Read the Full Article on Related Substances Vs Assay Test