Queer until proven cishet

(psychoville) mr jelly is bisexual but too overwhelmingly grumpy to date anyone

Doofus Rick (From Rick and Morty) is homoflexable

(Tales of Zestiria) Sorey is a gay trans boy, Mikleo is bi and nonbinary, Rose is a trans lesbian and Alisha is an ace lesbian :)

Jervis Tetch (Batman The Animated Series) is demisexual biromantic

(Monkey Island) Guybrush Threepwood is a trans man and is in the process of transitioning during the first game

Mami Tomoe is a trans girl

(brooklyn nine-nine) jake peralta is a biromantic bisexual trans man, amy santiago is a panromantic asexual demigirl, and rosa diaz is agender and a lesbian.

Big Boss from Metal Gear Solid is a trans boy and he's ready to CQC the SHIT out of the transphobes

morgan jones (the walking dead) is bisexual as hell

Yoosung Kim from Mystic Messenger is totally a trans guy.

(maniac mansion) Syd is gay, Micheal and Wendy are bisexual, and Razor is a lesbian trans woman

(m*a*s*h) hawkeye pierce is too bi to function

(hyperdrive) everyone is hyperdrive is bisexual, with becky sandstrom, dave vine and michael henderson having no preference, eduardo york and chloe teal having a preference for dudes and carl jeffers having a preference for women

(Persona 3) Makoto Yuki is a gay transmale, Fuuka yamagishi is aroace trans girl, Aigis is a nan0girl. Shinjiro is nonbinary with attraction to men.

(macbeth) lady macbeth is a trans dude

(Love Live) Eli Ayase is nonbinary and often binds their chest. Umi Sonoda is genderfluid and polyamorous. Nozomi Tojo is a cis lesbian. Nico Yazawa isn't really focused on labeling herself. She's not a girl, but doesn't really care to actually figure out what she is. Honoka Kousaka is pan and polyamorous. Kotori Minami is demi, polyamorous, and a trans girl. Rin Hoshizora is a trans girl, Hanayo Koizumi is cis and demi, and Maki Nishikino is a trans man or a demiboy.