That Mohs Tech

Right lower eyelid first stage at 2x with toluidine blue and hematoxylin and eosin

It's more obvious when you look in more closely, and in the depth, more so than in the epidermis.

This is a first stage Mohs layer

Basal cell carcinoma with H&E and toluidine blue

When I have cartilage, I put the slides on a hot plate on its lowest setting for 1-2 minutes (not much more!!! Or else the Oct bakes), then put them in 100% for 1 minute, a quick dip in water, and then I continue the H&E stain from the hematoxylin (or go into T. Blue)

Valentine's day themed mohs surgery

Notice the epidermal layer in the top right corner

A section of cartilage (second stage Mohs layer) stained with both toluidine blue and hematoxylin and eosin

Nodular basal cell carcinoma in a first stage Mohs layer

Nodular basal cell carcinoma stained with toluidine blue

BCC in a first stage Mohs layer

We had a big one today!

Showing off a nine stage Mohs case. Yes one case at one site that went on for 9 stages. Basal cell carcinoma

Just kidding, it is not normal for my cryostat to cry for help. I submitted a service ticket. Hopefully it's not serious.

Basal cell carcinoma in a first stage Mohs layer processed whole mount. The cancer is close to the bottom, on the right side of the separation/the 'nick' in the tissue

I put red ink on the face of the margin, then tell the pathology lab to place the red ink facing down. I also do them an extra, and after the specimen gets accessioned, i draw a map of how i grossed it, and how the inks are, and tell them to put the red ink facing down during embedding, and I write the accession number on it, and leave it by the embedding centers, so that way the embedders see it right away.

2x view