Jun 9
Basal cell carcinoma with H&E and toluidine blue
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Basal cell carcinoma with H&E and toluidine blue
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When your bosses send you flowers because your face looks like you need a #pickmeup #mohssurgery number 4 and 5 #notfun π€¦π»ββοΈπ€π #justcallmescarface #noselfieallowed
Infiltrative basal cell carcinoma in a first stage Mohs layer stained with H&E and toluidine blue at 2X
Notice the epidermal layer in the top right corner
Nodular basal cell carcinoma in a first stage Mohs layer
Great shot of nodular basal cell carcinoma at 2x in a first stage Mohs layer
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Right lower eyelid first stage at 2x with toluidine blue and hematoxylin and eosin
Changed my H&E stain from a progressive stain to a regressive stain by adding clarifier to the lineup (Using a Linistat stainer), and we got better contrast on the slides.
I already was diluting the eosin heavily with 100% alcohol, and I still am, and tried more water rinses and little tweaks in the protocol, and so the very best we had achieved was the progressive slide on the left, and after adding clarifier to the mix, you can see there's a slightly better contrast.
It's more obvious when you look in more closely, and in the depth, more so than in the epidermis.
Basal cell carcinoma, nice and obvious!
This is a first stage Mohs layer