#viralphd

Preparing ELISA plates for tomorrow. Its going to be a long day..good thing I'm going to have help 🦄🦋🙂 . . . . . . . . . . #enzymelinkedimmunosorbentassay #elisa #coatingplatewithprotein #biomedicalscience #immunology #vaccinedevelopment #gradschool #medicalresearch #hepatitiscvaccine #hepc #immuneresponse #antibodyresponse #sciart #pipetting #molecularbiology #colourchange #lab #science #laboratory #conical #science #96wellplate #toomuchpipetting #experiment #molecularbiology #stopsolution #immunology #scientist #phd #phdstudent #lablife #phdlife #viralphd

Strange feeling going to the lab and only have one experiment to do... Anyway have a great weekend everyone and keep on sciencing!! 🤓👨🏽‍🔬 . . . . . . . #bradford #research #elisa #bradfordassay #proteinassay #proteinassay #science #experiments #plates #biology #virology #biochemistry #chemistry #molecularbiology #bright #brightcolors #bluegreen #greenblue #sciencing #phdstudent #lablife #colourfull #colorful #scienceart #artsci #sciart #phd #viralphd #sciencing #happymothersday

Harvesting virus . Lysing cells using freeze-thawing method. I m freezing the cells in a dry ice/ethanol bath and then will thaw them at 37°C. . This method causes cells to swell and ultimately break as ice crystals form during the freezing process and then contract during thawing. . Will have to do this for about 4 cycles for efficient lysis. The next step will be to purify the virus from the cell lysate via ultracentrifugation . . . . . . . . #dryice #sciencing #freezing #viruspurification #dryicecloud #dryicefun #virus #phdlife #lablife #reseacher #molecularbiologylab #artofscience #sciart #molecularbiology #laboratory #vaccinology #science #scientist #phd #phdstudent #lablife #phdlife #viralphd #bluenitrilegloves #biology #experiments #immunology #phdlife #phdstudentlife

Its always a good feeling when an experiment finally works . I have been trying to optimize this ELISA for almost 4 months and it finally looks like its working! 🤓 . This is just the last step where I'm adding the stop solution before reading the plates . . . . . . . #enzymelinkedimmunosorbentassay #elisa #stopsolutioneffect #biomedicalscience #immunology #vaccinedevelopment #gradschool #medicalresearch #hepatitiscvaccine #hepc #immuneresponse #antibodyresponse #sciart #pipetting #molecularbiology #colourchange #lab #science #laboratory #conical #science #96wellplate #toomuchpipetting #experiment #molecularbiology #stopsolution #immunology #scientist #phd #phdstudent #lablife #phdlife #viralphd