#scanning electron microscope

A scanning electron microscope! People were free to sit down and play with this massive piece of equipment.

Would you happen to have a good source for close up spider toe bean pics for less hairy spiders? For art reference, I need details on the translucent jumping spider or similar.

I originality saw them in Biology of spiders by Rainer Foelix. There's a few pics on there. Most spiders are really hairy though, especially the feet. Here's an electron microscope pic of a jumping spider foot

A Journey into the World of Microscopy: From Humble Beginnings to High-Tech Magnification

The science of looking into the hidden invisible Microscopy has transformed our understanding of the world around us. It can explore the universe beyond the reach of our naked eyes, with complex cellular structures, red blood cells, viruses and other viruses and microorganisms taking on amazing perspectives

The history of the microscope is a fascinating story of human curiosity, scientific genius, and relentless exploration. From the humble beginnings of simple magnifying glasses to the sophistication of modern electronic microscopes, the invention of microscopes has shaped our understanding of the microscopic world

In the 1600s, Dutch opticians such as Hans and Zachary Janssen are credited with inventing the first microscope. Known for this hybrid microscope, many lenses were used to magnify objects up to 30 times.At the end of the 17th century, Antony van Leeuwenhoek, Dutch draper some changed our perception of thumbnails. Armed with a well-made single-lens microscope, and explored the hidden reaches of nature. In 1674, Leeuwenhoek discovered microorganisms in lake water, which he aptly named “animalcules”. His discovery laid the foundations of biology and inspired generations of scientists. This incredible feat allowed him to uncover a hidden universe – the first sightings of bacteria, red blood cells, and other microorganisms.

Formation of the scientific environment (17th-19th centuries): Leeuwenhoek’s discoveries boosted scientific research. Robert Hooke, an English scientist, established these developments. In 1665, his book "Micrographia" recorded his observations with a compound microscope. Notably, the term "cell" was coined by Hooke when he examined cork tissue, laying the foundation for cell biology.Microscope systems flourished throughout the 18th and 19th centuries Joseph Lister and other scientists addressed the limitations of the early lenses, introducing improvements that reduced image distortion.

Beyond the Limits of Light: The Beginning of the New Age (19th-20th century): As the 19th century progressed, the limitations of optical microscopy became apparent and scientists yearned for a tool which can go deeper into cells. This research culminated in the development of the electron microscope in the 1930s. The 20th century was revolutionary with the invention of the electron microscope. Unlike light microscopes, which use visible light, electron microscopes use electron beams to achieve much higher magnification.Formation of the scientific environment (17th-19th centuries): Leeuwenhoek’s discoveries boosted scientific research. Robert Hooke, an English scientist, established these developments. In 1665, his book "Micrographia" recorded his observations with a compound microscope. Notably, the term "cell" was coined by Hooke when he examined cork tissue, laying the foundation for cell biology.Microscope systems flourished throughout the 18th and 19th centuries Joseph Lister and other scientists addressed the limitations of the early lenses, introducing improvements that reduced image distortion.

Beyond the Limits of Light: The Beginning of the New Age (19th-20th century): As the 19th century progressed, the limitations of optical microscopy became apparent and scientists yearned for a tool which can go deeper into cells. This research culminated in the development of the electron microscope in the 1930s. The 20th century was revolutionary with the invention of the electron microscope. Unlike light microscopes, which use visible light, electron microscopes use electron beams to achieve much higher magnification.

In the 1930s, German experts Max Knoll and Ernst Ruska made the first electron microscope. This tool let us see tiny things like cells and even atoms by using electron beams, not light, getting images many times bigger. This cool invention showed us the tiny parts inside cells, viruses, and stuff too small to see before. The 1900s brought even more cool microscopes. New kinds like phase-contrast and confocal microscopy let scientists look at live cells without using stuff that could hurt them. Now, the world of looking at tiny things is getting even better. Today, we have high-tech microscopes that use computers and lasers. These let us see and even change tiny things in ways we never could before.

Modern Microscopy's Diverse Arsenal - Today, the field of microscopy boasts a diverse range of specialized instruments, each tailored to address specific scientific needs. Here's a glimpse into some remarkable examples:

Scanning Electron Microscope (SEM): Imagine a high-tech camera that captures images using a beam of electrons instead of light. That's the essence of a SEM. By scanning the surface of a sample with a focused electron beam, SEMs generate detailed information about its topography and composition. This makes them ideal for studying the intricate structures of materials like insect wings, microchips, and even pollen grains.

Transmission Electron Microscope (TEM): While SEMs provide exceptional surface detail, TEMs take us a step further. They function by transmitting a beam of electrons through a very thin sample, allowing us to observe its internal structure. TEMs are the go-to instruments for visualizing the intricate world of viruses, organelles within cells, and macromolecules like proteins.

Confocal Microscopy: Ever wished to focus on a specific layer within a thick biological sample and blur out the rest? Confocal microscopy makes this possible. It utilizes a laser beam to precisely illuminate a chosen plane within the sample, effectively eliminating information from out-of-focus regions. This allows researchers to create sharp, three-dimensional images of cells, tissues, and even small organisms.

Atomic Force Microscopy (AFM): This technique takes a completely different approach, venturing into the realm of physical interaction. AFM employs a tiny cantilever, akin to a microscopic feeler, to physically scan the surface of a sample. By measuring the minute forces between the cantilever and the sample's surface, AFM can map its topography at an atomic level. This provides invaluable insights into the properties of materials at an unimaginable scale, making it crucial for research in fields like nanotechnology and surface science.

Fluorescence Microscopy: Imagine illuminating a sample with specific wavelengths of light and observing it glowing in response. That's the essence of fluorescence microscopy. This technique utilizes fluorescent molecules or tags that bind to specific structures within a cell or tissue. When excited by light, these tags emit their own light, highlighting the target structures with remarkable clarity. This allows researchers to visualize specific proteins, DNA, or even pathogens within biological samples.

Super-resolution Microscopy (SRM): Overcoming the limitations imposed by the wavelength of light, SRM techniques like STED (Stimulated Emission Depletion) and PALM (Photoactivated Localization Microscopy) achieve resolutions surpassing the diffraction limit. This allows researchers to visualize structures as small as 20 nanometers, enabling the observation of intricate cellular machinery and the dynamics of individual molecules within living cells.

Cryo-Electron Microscopy (Cryo-EM): This powerful technique takes a snapshot of biological samples in their near-life state. Samples are rapidly frozen at ultra-low temperatures, preserving their native structure and minimizing damage caused by traditional fixation methods. Cryo-EM has been instrumental in determining the three-dimensional structures of complex molecules like proteins and viruses, providing crucial insights into their function and potential drug targets.

Correlative Microscopy: Combining the strengths of multiple microscopy techniques, correlative microscopy offers a comprehensive view of biological samples. For instance, researchers can utilize fluorescence microscopy to identify specific structures within a cell and then switch to electron microscopy to examine those structures in high detail. This integrated approach provides a deeper understanding of cellular processes and their underlying mechanisms.

Light Sheet Microscopy (LSM): Imagine illuminating a thin slice of a sample within a living organism. LSM achieves this feat by focusing a laser beam into a thin sheet of light, minimizing photobleaching and phototoxicity – damaging effects caused by prolonged exposure to light. This allows researchers to observe dynamic processes within living organisms over extended periods, providing valuable insights into cellular behavior and development.

Expansion Microscopy (ExM): This innovative technique physically expands biological samples by several folds while preserving their structural integrity. This expansion allows for better resolution and visualization of intricate cellular structures that would otherwise be difficult to distinguish using traditional microscopy methods. ExM holds immense potential for studying the organization and function of organelles within cells.

Scanning Near-Field Optical Microscopy (SNOM): This innovative technique pushes the boundaries of resolution by utilizing a tiny probe that interacts with the sample at an extremely close range. SNOM can not only image the surface features of a sample with exceptional detail but also probe its optical properties at the nanoscale. This opens doors for research in areas like material science and photonics, allowing scientists to study the behavior of light at the interface between materials.

X-ray Microscopy: Stepping outside the realm of light and electrons, X-ray microscopy offers unique capabilities. By utilizing high-energy X-rays, this technique can penetrate deep into samples, making it ideal for studying the internal structure of dense materials like bones and minerals. Additionally, it allows for the visualization of elements within a sample, providing valuable information about their distribution and composition.

Don’t let the purple hue fool you – this image captured by a scanning electron microscope is actually showing us mesoporous gold nanoparticles.

Photograph: Javeria Bashir/AIBN

Microscopes Simplified

Light Microscope

Fluorescence Microscope

Transmission Electron Microscope