Pathogenic variability among the twenty one isolates of Tilletia indica collected from 11 districts of Punjab and 4 from KPK. The response of some recent commercial varieties was also studied. Our study confirms the existence of pathotypes or aggressive types in isolates of T. indica in nature. As PB-25 was significantly more aggressive than all other isolates and showed moderately aggressive response with maximum mean coefficient of infection (6.03) while PB-03 was least aggressive. Based on pathogenic behavior on different varieties, isolates can be divided into two major groups. Among 12 commercial wheat varieties NIFA-Barsat, Punjab-2011, BARAS-09 and Seher-06 showed resistant to highly resistant response to all the isolates.