Taxus baccata is an endangered forest tree species with low regeneration. The highest Callus induction (96.67%) was occurred on ½ MS medium which had one-fourth nitrogen (KNO3, NH4NO3) supplemented with glutamine, 1 mg/l 2,4-D and 1 mg/l Kin from stem. The maximum callus size (80.67 mm2) was obtained from leaf culture on ½ MS medium in combination with glutamine, 2 mg/l NAA and 0.2 mg/l Kin. In order to observe cells meristematically, the tissue was transferred to the ½ MS medium supplemented with 0.4 mg/l 2,4-D and 3 BAP for 7-8 weeks. In micropropagation, adding activated charcoal (2 g/l) to the medium increased the average number of new leaves and shoot elongation. The maximum shoot elongation (2.66 cm) and growth new leaves were observed in the MS medium supplemented with 3 mg/l Kin after 1 month. The best Rooting of elongated shoots was obtained in the WPM medium without growth regulators.
