#fluroescence

Photogenic Fluorite Fluorite is the mineral that gives its name to the term “Fluorescence”, and these crystals of fluorite are fluorescing due to a UV lamp placed just outside the photo area. For a material to Fluoresce, it must be exposed to one type of electromagnetic radiation (light), absorb the energy from that light, and then give it back off as a different type of light.

Amazing Photos Reveal the Hidden Light of Undersea Life

Countstar® Rigel S6 Advanced Image Cytometer

PBMC cell count and Primary Cell Count

Cell Apoptosis

Cell Cycle

Surface Marker Analysis

GFP Transfection Efficiency

Optional FCS Express Software

Product Overview

Countstar® Rigel S6 is an automated image-based cell analysis platform with full-spectrum fluorescence Microscope, FCS Express software, analysis and reporting of both data and image.

Countstar® Rigel S6 equipped with 4 fluorescence channel, allow for 13 different fluorescent analysis combinations, support any dye of different wavelength.

Excitation light: 480nm, 525nm, 375nm, 620nm

Emission filter：460nm, 535nm, 580nm, 600LP, 665LP

Key Benefits of Fluroescence Cell Analyzer

All in one design- Integrated Computer/Touch screen

Automatic Detection 5 Samples in one single test sequence.

Open platform with maximum 4 fluorescence Channel

User Friendly Cell Analysis Protocols

Unrivaled "Fixed focus" patent, no manual focusing.

High Stability and Actuary

Powerful and user-friendly Data Management

GMP and 21 CFR part 11 ready

FCS Express Software

Product feature

Up to 4 fluorescent channel and one bright field analysis in the same time

13 different fluorescent analysis combinations

User Friendly Cell Analysis Protocols

Trypan blue Protocols: Obtain cell count, viability and concentration estimations based on trypan blue staining using a disposable consumable.

AO/PI Viability Protocol: Run two fluorescence color assays in disposable consumable to determine the percentages of live, dead cells and concentration in the presents of debris and unwanted nonnucleated cell types including red blood cells.

Affinityof Antibody:This assay run as Green color assays in FITC binding antibody to determine the affinity of the biosimilar drug.

Surface Marker Assay:Quantify specific cell populations based on surface marker expression (CD45+ CD4+, CD8 + MSC, CD56+ NK cells, etc.)

Cell Cycle:Propidium iodide (PI) is a nuclear staining dye that is frequently applied in measuring cell cycle. The protocol determining cellular DNA content in cell cycle analysis

Cell Apoptosis:Cell Apoptosis assay is a type used for determining the apoptosis percentage of cells by Annexin V-FITC/7-ADD staining method.

GFP transfection:The green fluorescent protein (GFP) exhibits bright green fluorescence when exposed to light in the blue to ultraviolet range. This protocol can analyze counting and percentage of GFP.

Celling Killing:Run three fluorescence color assays in disposable consumable to determine the CAR T/NK-Mediated Cytotoxicity using Tracer and Viability Dyes

GMP and 21 CFR part 11 ready

Make a detailed record of any changes, edits, deletions, adjustments, etc. Electronic records cannot be deleted, including administrators. Electronic records include date, time, user name, machine serial number, and user's actions

FCS Express Software

The optional DeNovoTMFCS Express software makes graphs touchable and customizable fluorescence channel boost your experiment reach. Countstar Rigel S6 together with FCS Express is able to analyze for the cell apoptosis, cell cycle, transfection, affinity of antibody, CD marker and etc.

Specifications

Technical Specifications

Model:

Countstar Rigel S6

Diameter range:

3μm ~ 180μm

Concentration range:

1×104 ~ 3×107/mL

Objective magnification:

5x

Imaging element:

1.4 megapixel, CCD camera

Excitation Light

480nm, 525nm, 375nm, 620nm

Emission Filter

460nm, 535nm, 580nm, 600LP, 665LP

USB

1×USB 3.0 1×USB 2.0

Storage:

500GB

Power supply:

110–230 V/AC, 50/60Hz

Screen:

10.4 inch touchscreen

Weight:

13kg (28lb)

Size (W X D X H):

Machine: 254×303×453mm Package size: 430×370×610mm

Operating temperature:

10℃ ~ 40℃

Working humidity:

20% ~ 80%

Applications Dual-Fluorescence Viability

Acridine orange (AO) and Propidium iodide (PI) are nuclear nucleic acid binding dyes. The analysis excludes cell fragments, debris and artifacts particles as well as undersized events such as platelets, giving a highly accurate result. In conclusion, the Countstar system can be used for every step of the cell manufacturing process.

T/NK cell mediated cytotoxicity

By labeling the target tumor cells with CFSE or transfect with GFP. Hoechst 33342 is used for stain all cells (both T cells and tumor cells), alternatively, target tumor cells can be stained with CFSE, PI is used for stain the dead cells (both T cells and tumor cells). This staining strategy allows for the discriminate of different cells.

Cell Apoptosis

The different phases of apoptosis are segmented into an early, middle and late stage. Specified indicators can document the single stages of apoptosis. The Countstar® Rigel S6 offers a visualized image-based solution and analyzes all of these steps with pre-loaded protocols in depth.

Cell Cycle

Countstar Rigel S6 enables users to get the result of a cell cycle quickly and accurately. Counstar Rigel S6 can analyze proportion of cell in different phase of the cell cycle.

Surface Maker Analysis

A lymphocyte subsets analysis is a typical experiment performed in cell related research fields and various diseases diagnosis. Countstar® Rigel S6 offers a faster and easier way to make immune cell typing more efficient. With visible cell images and powerful data analysis.

Affinity of antibody

Detect affinity of antibody in cell level is an important indicator of monoclonal antibody detection using immunofluorescence method.The Countstar® Rigel S6 offers a rapid, direct and reliable evaluating method for the affinity of antibody detection in antibody drug screening

GFP Transfection Efficiency

In cell and molecular biology, the GFP gene is frequently used as a reporter of expression. Currently, scientists are commonly using the fluorescent microscopes or flow cytometers to analyze the transfection efficiency of mammalian cells. But Flow cytometer requires a high-qualified and experienced operator. While Countstar Rigel S6 enables users to get the result of a transfection efficiency assay quickly and accurately.

Fluorapatite, fluorescing

One of the more eerie seeming properties of some minerals is their ability to glow in the dark when submerged in ultraviolet light, fluorite (after which the phenomenon was named when discovered) being the most obvious example. Atoms within the crystal lattice get excited by the high energy rays, and their electrons jump up with this extra energy to a different shell. They can't hold on to it though, and shed it as they drop back in the form of light at visible wavelengths.

The 5.5 x 4.1 x 1.8 cm example in the photo is a colour zoned fluorine rich apatite (see http://bit.ly/2akYWkg for a detailed discusion) from the Panasqueira Mines of Portugal. As the crystal grew, a pulse of the fluid from which it precipitated was charged with a colour causing impurity which created the green zoning. As you can see, the electrons in this zone of the crystal absorb and shed the energy differently, with higher energy blue wavelengths coming off the white portion of the crystal, and a lovely yellowish green from the coloured portion.

Loz

More Nobel Prize things! :D

BREAKING NEWS:

The Nobel Prize in Chemistry has been awarded to Eric Betzig, Stefan W. Hell and William E. Moerner "for the development of super-resolved fluorescence microscopy".