Countstar

The body's immune system is an effective weapon against disease, and immunotherapy of cancer cell research is the current frontier in the treatment of cancer and other diseases.

  Recently, researchers at Notre Dame University have identified specific features of the reaction of two distinct peptide antigens to a T-cell receptor (TCR), which provides new clues to optimizing molecular structure for the development of immunotherapy of cancer cell research.

 The results were published in the latest issue of Nature Chemical Biology.

  cancer cell research

  'We found that T-cell receptors might be more cross-reactive than we thought they were.' Said the lead author Timothy Riley, a former graduate student assistant at Baker and now co-founder, scientist and director of the startup Structured Immunity, which incubated through Notre Dame's IDEA center to reduce the risk of  early exposure of immunotherapy.

  T cells, a subtype of white blood cells, are responsible for sensing whether the body is healthy or infected, but they often ignore the presence of cancer cells in the body. In T-cell immunotherapy, cancer cells are allowed to look for and destroy specific cells by adding receptor molecules that are recognized by the body's immune system. Although this treatment is effective in some cases, it often affects healthy cells. Therefore, researchers hope to predict responsiveness and reduce nonspecific responses as far as possible.

  In this study, the authors found that a TCR molecule called DMF5 binds to a variety of different peptides. Two different peptide antigens, which bind to DMF5, have the same effect in stimulating receptors and inducing immune responses. 'As long as it happens, its working style is not important,' the author said.

Countstar® Rigel S6 Advanced Image Cytometer

PBMC cell count and Primary Cell Count

Cell Apoptosis

Cell Cycle

Surface Marker Analysis

GFP Transfection Efficiency

Optional FCS Express Software

Product Overview

Countstar® Rigel S6 is an automated image-based cell analysis platform with full-spectrum fluorescence Microscope, FCS Express software, analysis and reporting of both data and image.

Countstar® Rigel S6 equipped with 4 fluorescence channel, allow for 13 different fluorescent analysis combinations, support any dye of different wavelength.

Excitation light: 480nm, 525nm, 375nm, 620nm

Emission filter：460nm, 535nm, 580nm, 600LP, 665LP

Key Benefits of Fluroescence Cell Analyzer

All in one design- Integrated Computer/Touch screen

Automatic Detection 5 Samples in one single test sequence.

Open platform with maximum 4 fluorescence Channel

User Friendly Cell Analysis Protocols

Unrivaled "Fixed focus" patent, no manual focusing.

High Stability and Actuary

Powerful and user-friendly Data Management

GMP and 21 CFR part 11 ready

FCS Express Software

Product feature

Up to 4 fluorescent channel and one bright field analysis in the same time

13 different fluorescent analysis combinations

User Friendly Cell Analysis Protocols

Trypan blue Protocols: Obtain cell count, viability and concentration estimations based on trypan blue staining using a disposable consumable.

AO/PI Viability Protocol: Run two fluorescence color assays in disposable consumable to determine the percentages of live, dead cells and concentration in the presents of debris and unwanted nonnucleated cell types including red blood cells.

Affinityof Antibody:This assay run as Green color assays in FITC binding antibody to determine the affinity of the biosimilar drug.

Surface Marker Assay:Quantify specific cell populations based on surface marker expression (CD45+ CD4+, CD8 + MSC, CD56+ NK cells, etc.)

Cell Cycle:Propidium iodide (PI) is a nuclear staining dye that is frequently applied in measuring cell cycle. The protocol determining cellular DNA content in cell cycle analysis

Cell Apoptosis:Cell Apoptosis assay is a type used for determining the apoptosis percentage of cells by Annexin V-FITC/7-ADD staining method.

GFP transfection:The green fluorescent protein (GFP) exhibits bright green fluorescence when exposed to light in the blue to ultraviolet range. This protocol can analyze counting and percentage of GFP.

Celling Killing:Run three fluorescence color assays in disposable consumable to determine the CAR T/NK-Mediated Cytotoxicity using Tracer and Viability Dyes

GMP and 21 CFR part 11 ready

Make a detailed record of any changes, edits, deletions, adjustments, etc. Electronic records cannot be deleted, including administrators. Electronic records include date, time, user name, machine serial number, and user's actions

FCS Express Software

The optional DeNovoTMFCS Express software makes graphs touchable and customizable fluorescence channel boost your experiment reach. Countstar Rigel S6 together with FCS Express is able to analyze for the cell apoptosis, cell cycle, transfection, affinity of antibody, CD marker and etc.

Specifications

Technical Specifications

Model:

Countstar Rigel S6

Diameter range:

3μm ~ 180μm

Concentration range:

1×104 ~ 3×107/mL

Objective magnification:

5x

Imaging element:

1.4 megapixel, CCD camera

Excitation Light

480nm, 525nm, 375nm, 620nm

Emission Filter

460nm, 535nm, 580nm, 600LP, 665LP

USB

1×USB 3.0 1×USB 2.0

Storage:

500GB

Power supply:

110–230 V/AC, 50/60Hz

Screen:

10.4 inch touchscreen

Weight:

13kg (28lb)

Size (W X D X H):

Machine: 254×303×453mm Package size: 430×370×610mm

Operating temperature:

10℃ ~ 40℃

Working humidity:

20% ~ 80%

Applications Dual-Fluorescence Viability

Acridine orange (AO) and Propidium iodide (PI) are nuclear nucleic acid binding dyes. The analysis excludes cell fragments, debris and artifacts particles as well as undersized events such as platelets, giving a highly accurate result. In conclusion, the Countstar system can be used for every step of the cell manufacturing process.

T/NK cell mediated cytotoxicity

By labeling the target tumor cells with CFSE or transfect with GFP. Hoechst 33342 is used for stain all cells (both T cells and tumor cells), alternatively, target tumor cells can be stained with CFSE, PI is used for stain the dead cells (both T cells and tumor cells). This staining strategy allows for the discriminate of different cells.

Cell Apoptosis

The different phases of apoptosis are segmented into an early, middle and late stage. Specified indicators can document the single stages of apoptosis. The Countstar® Rigel S6 offers a visualized image-based solution and analyzes all of these steps with pre-loaded protocols in depth.

Cell Cycle

Countstar Rigel S6 enables users to get the result of a cell cycle quickly and accurately. Counstar Rigel S6 can analyze proportion of cell in different phase of the cell cycle.

Surface Maker Analysis

A lymphocyte subsets analysis is a typical experiment performed in cell related research fields and various diseases diagnosis. Countstar® Rigel S6 offers a faster and easier way to make immune cell typing more efficient. With visible cell images and powerful data analysis.

Affinity of antibody

Detect affinity of antibody in cell level is an important indicator of monoclonal antibody detection using immunofluorescence method.The Countstar® Rigel S6 offers a rapid, direct and reliable evaluating method for the affinity of antibody detection in antibody drug screening

GFP Transfection Efficiency

In cell and molecular biology, the GFP gene is frequently used as a reporter of expression. Currently, scientists are commonly using the fluorescent microscopes or flow cytometers to analyze the transfection efficiency of mammalian cells. But Flow cytometer requires a high-qualified and experienced operator. While Countstar Rigel S6 enables users to get the result of a transfection efficiency assay quickly and accurately.

Countstar® Rigel S5 Advanced Image Cytometer

PBMC and Primary Cell Counting

Cell Apoptosis

Cell Cycle

Surface Marker Analysis

GFP Transfection Efficiency

FCS Express Software

Product Overview

The Countstar Rigel S5 system increases the PE channel on the basis of Rigel S3, and can carry out multi-color fluorescence experiments in addition to rapid cell viability and concentration measurement, including cell apoptosis (Annexin V/PI/DAPI), cell transfection (GFP/YFP/RFP), CD marker experiment (DAPI/FITC/PE), and cell killing (Hoechst T33342/Calcein-AM/PI) and so on.

Excitation light: 375nm, 480nm, 525nm

Emission filter：460nm, 535nm, 580nm, 600LP

Key Benefits

All in one design- Integrated Computer/Touch scree

Automatic Detection 5 Samples in one single test sequence.

User Friendly Cell Analysis Protocols

Unrivaled "Fixed focus" patent, no manual focusing.

High Stability and Actuary

Powerful and user-friendly Data Management

GMP and 21 CFR part 11 ready

FCS Express Software

Product feature

SmartThe BioApps of the Countstar Rigel S3 System simplify routine cell lab tasks while providing high quality scientific data.

Countstar S3 comes with three BioApps to simplify and automate the routine cell count, viability and cytotoxicity tasks.

AO/PI Viability Protocol: Run two fluorescence color assays in disposable consumable to determine the percentages of live, dead cells and concentration in the presents of debris and unwanted nonnucleated cell types including red blood cells.

Affinity of Antibody: This assay run as Green color assays in FITC binding antibody to determine the affinity of the biosimilar drug.

Surface Marker Assay: Quantify specific cell populations based on surface marker expression (CD45+ CD4+, CD8 + MSC, CD56+ NK cells, etc.)

Cell Cycle: Propidium iodide (PI) is a nuclear staining dye that is frequently applied in measuring cell cycle. The protocol determining cellular DNA content in cell cycle analysis

Cell Apoptosis: Cell Apoptosis assay is a type used for determining the apoptosis percentage of cells by Annexin V-FITC/7-ADD staining method.

GFP transfection: The green fluorescent protein (GFP) exhibits bright green fluorescence when exposed to light in the blue to ultraviolet range. This protocol can analyze counting and percentage of GFP.

Celling Killing: Run three fluorescence color assays in disposable consumable to determine the CAR T/NK-Mediated Cytotoxicity using Tracer and Viability Dyes

Trypan blue Protocols: Obtain cell count, viability and concentration estimations based on trypan blue staining using a disposable consumable.

Three step process

The Countstar S3 System is designed to take you from sample to result in one go, simplifying your work and allowing for increased productivity and efficiency.

Reproducible and Accurate

Countstar S3 system can automatic detect 5 samples at one time, it will be more efficiency and affordable, with integrated patent “fixed focus”, the results would be more stable and reliable.

Touch Screen with Flexible Experiment Assay Management System

“App-like" interface. Allow to add multiple Experiment Assays for different operators

FCS Express Software

The optional De NovoTM FCS Express software makes graphs touchable and customizable fluorescence channel boost your experiment reach. Countstar Rigel S5 together with FCS Express is able to analyze for the cell apoptosis, cell cycle, transfection, affinity of antibody, CD marker and etc.

GMP ready

The Countstar S5 system is designed to meet the modern pharmaceutical requirements. The software complies with 21 CFR part 11. Key features include control of user access and data export, and secure audit trail. At the same time, we also supply IQ/OQ service and PQ support to help customer build a reliable system.

Specifications

Technical Specifications

Model:

Countstar Rigel S5

Diameter range:

3μm ~ 180μm

Concentration range:

1×104 ~ 3×107/mL

Objective magnification:

5x

Imaging element:

1.4 megapixel ,CCD camera

Excitation Light

375nm, 480nm, 525nm

Emission Filter

460nm, 535nm, 580nm, 600LP

USB

1×USB 3.0 1×USB 2.0

Storage:

500GB

Power supply:

110–230 V/AC, 50/60Hz

Screen:

10.4 inch touchscreen

Weight:

13kg (28lb)

Size (W X D X H):

Machine: 254×303×453mm Package size: 430×370×610mm

Operating temperature:

10℃ ~ 40℃

Working humidity:

20% ~ 80%

Applications Dual-Fluorescence Viability

Acridine orange (AO) and Propidium iodide (PI) are nuclear nucleic acid binding dyes. The analysis excludes cell fragments, debris and artifacts particles as well as undersized events such as platelets, giving a highly accurate result. In conclusion, theCountstarsystem can be used for every step of the cell manufacturing process.

T/NK cell mediated cytotoxicity

By labeling the target tumor cells with CFSE ortransfectwith GFP. Hoechst 33342 is used for stain all cells (both T cells and tumor cells), alternatively, target tumor cells can be stained with CFSE, PI is used forstainthe dead cells (both T cells and tumor cells). This staining strategy allows for the discriminate of different cells.

Cell Apoptosis

The different phases of apoptosis are segmented into an early, middle and late stage. Specified indicators can document the single stages of apoptosis. The Countstar® Rigel S5 offers a visualized image-based solution and analyzes all of these steps with pre-loaded protocols in depth.

Cell Cycle

CountstarRigel S5 enables users to get the result of a cell cycle quickly and accurately.CounstarRigel S5 can analyzeproportionof cell indifferentphase of the cell cycle.

Surface Maker Analysis

A lymphocyte subsets analysis is a typical experiment performed incell relatedresearch fields and various diseases diagnosis. Countstar® Rigel S5 offers a faster and easier way to make immune cell typing more efficient. With visible cell images and powerful data analysis

Affinity of antibody

Detect affinity of antibody in cell level is an important indicator of monoclonal antibody detection using immunofluorescence method.TheCountstar® Rigel S5 offers a rapid, direct and reliable evaluating method for the affinity of antibody detection in antibody drug screening

GFP Transfection Efficiency

In cell and molecular biology, the GFP gene is frequently used as a reporter of expression. Currently, scientists are commonly using the fluorescent microscopes or flow cytometers to analyze the transfection efficiency of mammalian cells. But Flow cytometry requires a high-qualified and experienced operator. WhileCountstarRigel S5 enables users to get the result of a transfection efficiency assay quickly and accurately.

Countstar® Rigel S3

Fluorescence Cell Analyzer

CAR-T Cell cell mediated Cytotoxicity Studies

Phenotypic Charaterization of CAR-T cells

Viability and Concentration assay for :

1. Quality control of original blood samples

2. PBMC isolation efficiency tests

3. Cultured status of incubated T-lymphocytes

4. Process Monitoring of modified CAR-T cells

5. Final Product released control.

Product Overview

The Countstar S3 System combines the three fluorescence channels plus a bright field digital microscope, an image cytometer, and a cell counter in a single bench-top instrument. This application-driven, compact, automated cell imaging system provides an all-in-one solution for cell counting, cell viability and T/NK cell mediated cytotoxicity through the use of preconfigured biological applications (BioApps). They are designed to simplify routine cell lab tasks，which leaves you time to focus on the research!

Excitation light: 375nm, 480nm, 525nm

Emission filter：460nm, 535nm, 600LP

Key Benefits

All in one design- Integrated Computer/Touch screen

Automatic Detection 5 Samples in one single test sequence.

Open platform with maximum 4 fluorescence Channel

User Friendly Cell Analysis Protocols

Unrivaled "Fixed focus" patent, no manual focusing.

High Stability and Actuary

Powerful and user-friendly Data Management

GMP and 21 CFR part 11 ready

FCS Express Software

Best Fluorescence Cell Viability Counting with Countstar® Rigel S3 Fluorescence Cell Analyzer

Product feature

Up to 4 fluorescent channel and one bright field analysis at the same time

13 different fluorescent analysis combinations

User Friendly Cell Analysis Protocols

Trypan blue Protocols: Obtain cell count, viability and concentration estimations based on trypan blue staining using a disposable consumable.

AO/PI Viability Protocol: Run two fluorescence color assays in disposable consumable to determine the percentages of live, dead cells and concentration in the presents of debris and unwanted nonnucleated cell types including red blood cells.

Affinity of Antibody: This assay run as Green color assays in FITC binding antibody to determine the affinity of the biosimilar drug.

Surface Marker Assay: Quantify specific cell populations based on surface marker expression (CD45+ CD4+, CD8 + MSC, CD56+ NK cells, etc.)

Cell Cycle: Propidium iodide (PI) is a nuclear staining dye that is frequently applied in measuring cell cycle. The protocol determining cellular DNA content in cell cycle analysis

Cell Apoptosis: Cell Apoptosis assay is a type used for determining the apoptosis percentage of cells by Annexin V-FITC/7-ADD staining method.

GFP transfection: The green fluorescent protein (GFP) exhibits bright green fluorescence when exposed to light in the blue to ultraviolet range. This protocol can analyze counting and percentage of GFP.

Celling Killing: Run three fluorescence color assays in disposable consumable to determine the CAR T/NK-Mediated Cytotoxicity using Tracer and Viability Dyes

GMP and 21 CFR part 11 ready

Make a detailed record of any changes, edits, deletions, adjustments, etc. Electronic records cannot be deleted, including administrators. Electronic records include date, time, user name, machine serial number, and user's actions

FCS Express Software

The optional De NovoTM FCS Express software makes graphs touchable and customizable fluorescence channel boost your experiment reach. Countstar FL together with FCS Express is able to analyze for the cell apoptosis, cell cycle, transfection, affinity of antibody, CD marker and etc.

Specifications

Technical Specifications

Model:

Countstar Rigel S3

Diameter range:

3μm ~ 180μm

Concentration range:

1×104 ~ 3×107/mL

Objective magnification:

5x

Imaging element:

1.4 megapixel ,CCD camera

Excitation Light

375nm, 480nm, 525nm

Emission Filter

460nm, 535nm, 600LP

USB

1×USB 3.0 1×USB 2.0

Storage:

500GB

Power supply:

110–230 V/AC, 50/60Hz

Screen:

10.4 inch touchscreen

Weight:

13kg (28lb)

Size (W X D X H):

Machine: 254×303×453mm Package size: 430×370×610mm

Operating temperature:

10℃ ~ 40℃

Working humidity:

20% ~ 80%

Applications

Dual-Fluorescence Viability:

Acridine orange (AO) and Propidium iodide (PI) are nuclear nucleic acid binding dyes. The analysis excludes cell fragments, debris and artifacts particles as well as undersized events such as platelets, giving a highly accurate result. In conclusion, the Countstar system can be used for every step of the cell manufacturing process.

T/NKcell mediatedcytotoxicity

By labeling the target tumor cells with CFSE or transfect with GFP. Hoechst 33342 is used for stain all cells (both T cells and tumor cells), alternatively, target tumor cells can be stained with CFSE, PI is used for stain the dead cells (both T cells and tumor cells). This staining strategy allows for the discriminate of different cells.

Cell Apoptosis

The different phases of apoptosis are segmented into an early, middle and late stage. Specified indicators can document the single stages of apoptosis. The Countstar® FL offers a visualized image-based solution and analyzes all of these steps with pre-loaded protocols in depth.

Cell CycleCountstar Rigel S6 enables users to get the result of a cell cycle quickly and accurately. Counstar Rigel S6 can analyze proportion of cell in different phase of the cell cycle.

Surface Maker Analysis

A lymphocyte subsets analysis is a typical experiment performed in cell related research fields and various diseases diagnosis. Countstar® Rigel S6 offers a faster and easier way to make immune cell typing more efficient. With visible cell images and powerful data analysis

Affinity of antibody

Detect affinity of antibody in cell level is an important indicator of monoclonal antibody detection using immunofluorescence method.The Countstar® Rigel S6 offers a rapid, direct and reliable evaluating method for the affinity of antibody detection in antibody drug screening

GFP Transfection Efficiency

In cell and molecular biology, the GFP gene is frequently used as a reporter of expression. Currently, scientists are commonly using the fluorescent microscopes or flow cytometers to analyze the transfection efficiency of mammalian cells. But Flow cytometer requires a high-qualified and experienced operator. While Countstar Rigel S6 enables users to get the result of a transfection efficiency assay quickly and accurately.

Countstar® Rigel S2 Fluorescence Cell Analyzer

Precise cell analysis for:

PBMCs, whole blood, Car-T

Stem Cell Analysis

Splenocytes

Monocytes

And Other Primary Cells

Product Overview

The Countstar S2 System combines the two fluorescence channels plus a bright field digital microscope, an image cytometer, and a cell counter in a single bench-top instrument. This application-driven, compact, automated cell imaging system provides an all-in-one solution for cell counting, cell viability and Cell transfection efficiency through the use of preconfigured biological applications (BioApps). They are specifically optimized for analysis of primary cells from peripheral blood, including PBMC, CAR-T, NK cell and MSC, which commonly used in cell therapy.

Excitation light: 480nm, 525nm

Emission filter：535nm, 600LP

Key Benefits

Automatic detecting 5 samples for one time

GMP and 21 CFR Part 11 ready

Multi channel, multi functions

Simple, effortless operation

Maintenance and service free

All in one design- Touch screen user interface

User friendly and flexible software

Countstar Rigel S2 fluorescence analyzer feature

Smart

The BioApps of the Countstar Rigel S2 System simplify routine cell lab tasks while providing high quality scientific data.

Countstar S2 comes with three BioApps to simplify and automate the routine cell count, viability and cytotoxicity tasks.

Trypan blue BioApp: Obtain cell count, viability and concentration estimations based on trypan blue staining using a disposable consumable.

AO/PI Viability BioApp: Run two fluorescence color assays in disposable consumable to determine the percentages of live, dead cells and concentration in the presents of debris and unwanted nonnucleated cell types including red blood cells.

GFP transfection: The green fluorescent protein (GFP) exhibits bright green fluorescence when exposed to light in the blue to ultraviolet range. This protocol can analyze counting and percentage of GFP.

Three step process

The Countstar S2 System is designed to take you from sample to result in one go, simplifying your work and allowing for increased productivity and efficiency.

Reproducible and Accurate

Countstar S2 system can automatic detect 5 samples at one time, it will be more efficiency and affordable, with integrated patent “fixed focus”, the results would be more stable and reliable.

Touch Screen with Flexible Experiment Assay Management System

“App-like" interface. Allow to add multiple Experiment Assays for different operators

GMP ready

The Countstar S2 system is designed to meet the modern pharmaceutical requirements. The software complies with 21 CFR part 11. Key features include control of user access and data export, and secure audit trail. At the same time, we also supply IQ/OQ service and PQ support to help customer build a reliable system.

Specifications

Technical Specifications

Model:

Countstar Rigel S2

Diameter range:

3μm ~ 180μm

Concentration range:

1×104 ~ 3×107/mL

Objective magnification:

5x

Imaging element:

1.4 megapixel ,CCD camera

Excitation Light

480nm, 525nm

Emission Filter

535nm, 600LP

USB

1×USB 3.0 1×USB 2.0

Storage:

500GB

Power supply:

110–230 V/AC, 50/60Hz

Screen:

10.4 inch touchscreen

Weight:

13kg (28lb)

Size (W X D X H):

Machine: 254×303×453mm Package size: 430×370×610mm

Operating temperature:

10℃ ~ 40℃

Working humidity:

20% ~ 80%

Applications

Monitoring the Quality of CAR-T Production during the whole Process

The immuno therapy, such as CAR-T involves the collection of peripheral blood mononuclear cells (PBMC) from the patient, then enrichment of the desired T cell subset followed by genetic modification to create engineered CAR-T cells. The CAR-T cells are expanded in large-scale and then infused back into the patient. With the Countstar S2 system it is possible to continuously monitor the viability and concentration every step of the entire process of manufacturing CAR-T cells under GMP.

Dual-Fluorescence Viability

Acridine orange (AO) and Propidium iodide (PI) are nuclear nucleic acid binding dyes. AO can penetrate to both dead and live cells and stains the nucleated cells to generate green fluorescence. PI can stain the dead nucleated cells with compromised membranes and generate red fluorescence. The analysis excludes cell fragments, debris and artifacts particles as well as undersized events such as platelets, giving a highly accurate result. In conclusion, the Countstar S2 system can be used for every step of the cell manufacturing process.

GFP Transfection Efficiency

In cell and molecular biology, the GFP gene is frequently used as a reporter of expression. Currently, scientists are commonly using the fluorescent microscopes or flow cytometers to analyze the transfection efficiency of mammalian cells. But Flow cytometer requires a high-qualified and experienced operator. While Countstar FL enables users to get the result of a transfection efficiency assay quickly and accurately.

Trypan blue viability and concentration

Trypan blue viability and concentration assay also can be available on Countstar S2. Shown here, are the enlarged representative images of cell line. Since all of the cells were stained with trypan blue, we show the stained trypan blue image as well as the Countstar S2 counted image.

Countstar® Altair Advanced Cell Analyzer for Industry

Product Overview

The Countstar® Altair is designed based on the classic Trypan Blue exclusion principle, integrating the advanced “fix focus” optical imaging bench, the most advanced cell recognition technologies, and software algorithms. Enable to get the information of cell concentration, viability, aggregation rate, roundness, and diameter distribution by one run.

To meet the cGMP regulation, Countstar® Atair software, equipped with a powerful data management function, offers a 4-level user access control feature, e-signatures, and log files that is in compliance with the FDA 21 CFR Part 11 regulations.

Key Benefits of Countstar Full Automated Cell Counter

All in one design, integrated Computer/touch screen

Automatic Detection 5 Samples in one single test sequence, 2.5 min /5 sample.

Unrivaled "Fixed focus" patent, no manual focusing.

Powerful and user-friendly Data Management

Security User Login and 4 level user access management

FDA CFR 21 Part11 Ready

IQ/OQ/PQ Service

Product feature Automated, Fast and Easy Operation

Only need three steps to get the test result.

Touch Screen with Flexible Experiment Assay Management System

“App-like" interface. Allow to add multiple Experiment Assays for different operators

Most Advanced Imaging Technology

1. Unrivaled "Fixed focus" patent

There is no need for the user to focus under any circumstances: replacing the slide, changing the sample slot, different regions of interest selected. Results are highly reproducible and an interference by the operator is excluded

2. 5 megapixel industrial colour camera to provide high resolution images

Countstar Altair

3. Our protected image recognition algorithms analyze more than 20 single parameters of each object classified

Powerful and User-Friendly Data Management

FDA 21 CFR Part 11

Make a detailed record of any changes, edits, deletions, adjustments, etc. Electronic records cannot be deleted, including administrators. Electronic records include date, time, username, machine serial number, and user's actions

Instrument Stability Test (IST)

Shanghai RuiYu BioTech has established a comprehensive validation plan that test and monitor the stability and accuracy of the result of Countstar Mars to guarantee the data is reliable in the daily use. Shanghai RuiYu BioTech has deigned an examination program (Instrument Stability Test, IST) to make sure that performance of instruments meets the requirements of cGMP regulated environments. IST will examine and calibrate the instrument once in a period of time to prove that the result measured by this instrument remains accurate and stable after years of use.

Specifications

Technical Specifications

Model:

Countstar Altair

Diameter range:

3μm ~ 180μm

Concentration range:

1×104 ~ 3×107/mL

Objective magnification:

2.5x

Imaging element:

5 megapixel,CMOS camera

USB

1×USB 3.0 1×USB 2.0

Storage:

500GB

Power supply:

110–230 V/AC, 50/60Hz

Screen:

10.4 inch touchscreen

Weight:

13kg (28lb)

Size (W X D X H):

Machine: 254×303×453mm Package size: 430×370×610mm

Operating temperature:

10℃ ~ 40℃

Working humidity:

20% ~ 80%

Applications

Development of bioprocessing in antibody Production during the whole Process

During the whole process from antibody R&D to production, there are many steps need to carry out cell based assay to evaluate the process or quality control. Such as the total cell concentration and viability will define the status of the cell culture.

Trypan blue cell counting and viability

Countstar is applicable to cells with diameter of 5-180um, like mammalian cell, insect cell, and some planktons.

Cell Size Analysis

The change of cell size is a key feature and is commonly measured in cell research. Normally it will be measured in these experiments: cell transfection, drug test and cell activation assays.

Countstar Biotech VS Countstar Altair

Countstar Altair Data easily comparable with a Countstar Biotech

Countstar® BioTech Automated Cell Counter

Accurate cell analysis for:

Trypan blue Cell Counting

Aggregated Cell Analysis

Cell Diameter Analysis

Cultivation time chart (CTC)

Product Overview

The Countstar® is designed based on the classic trypan blue staining for cell viability principle, integrating the advanced “fix focus” optical imaging work bench and the most advanced cell recognition technologies and software algorithms. Countstar BioTech not only provides the cell concentration and viability, but also provides the average diameter and average circularity, and showing the diameter range and aggregation of cells in histograms etc.

Key Benefits

“Fixed Focus”– No manual focus adjustment needed

Fast testing time – less than 20s

Data easily comparable with a hemocytometer

Maximum loaded 5 samples in one slide

Accurate and high reproducibility result – Intra-assay & Inter-assay CV <5%

Maintenance and service free

Powerful software and data management System

IQ/OQ validation and PQ support

Product feature Easy and fast:

Within 20 seconds through three steps with one button

Cost-efficient Consumables:

Loaded 5 sample in one slide             Individual Package

Imaging Technology:

5-megapixel color imaging and a wide sample collection range ensures clear and accurate visualization

Accuracy and Reliability:

Guaranteed by “Fixed Focus” technology, high resolution imaging, larger observation area and advanced algorithms.

Countstar VS Hemocytometer

Powerful data analysis and management System:

1. Intelligence search, Coded lock, security user login and sustainable data management etc., to keep the cell quality and data safety.

2. Countstar software system provides cultivation time chart (CTC), overlay analysis and other statistical and analytical functions

3. Diversity of Data Formats: PDF, EXCEL, JPEG；Automated PDF reports

4. Sustainable Data Management

User, Time, Parameter, Result……

Specifications

Technical Specifications

Model:

IC 1000

Test Item：

Concentration, Viability, Diameter, aggregation etc.

Sample Density：

1x104 - 3x107/ml

Sample Diameter：

5-180μm

Imaging element:

5 Megapixel，CMOS camera

Objective magnification:

2.5 X

Sample Volume：

20μL

Test Time：

<20s

Output：

JPEG/PDF/EXCE

Applications 1. Trypan blue cell counting and viability

Countstar is applicable to cells with diameter between 5-180um, like mammalian cell, insect cell, and some planktons.

2. Aggregated Cell Analysis

Some primary cells or subculture cells are prone to aggregate when poor culture state or excessive digestion, thus causing great difficulty in the counting of cells. With the Aggregation Calibration Function, Countstar® can realize a stimulation calculation of aggregations to ensure accurate cell counting and obtain the aggregation rate and the aggregation histogram, thus providing basis for experimenters to judge the state of cells.

Countstar can count the aggregated cells one by one.

3. Cell Size Analysis

The change of cell size is a key feature and is commonly measured in cell research. Normally it will be measured in these experiments: cell transfection, drug test and cell activation assays.

4. Overlay Analysis of the Cell Growth Curve

In cell culture, the study of pharmacology and toxicology often requires the overlay analysis of multiple growth curves in order to find the optimal culture conditions or the best dosage. Countstar® can directly call up multiple growth curves for comparative analysis.

Countstar® BioMed Professional Immune Cell Counter

1. Trypan blue Cell Counting

2. Immune Cell Counter

3. Cell Diameter Analysis

4. Cultivation time chart (CTC)

Product Overview

In order to meet the requirement of cell therapy institutes, RuiYu developed the Countstar BioMed. It is applicable to cells with diameters ranging from 2-180μm, and realizes an accurate and reliable counting of cells commonly seen in clinical treatment such as lymphocytes, dendritic cells and other stem cells. Providing a series of solution from cell counter, cell analysis, data storage and manage to data output.

Key Benefits

More Wider Test Range: Cell diameter: 2-180μm；

"Fixed Focus" – No manual focus adjustment needed

One equipment code specific to each measurement;

Powerful software and data management System;

E-signature and 4-level User Access Management;

IQ/OQ validation and PQ support

Product feature

Keeping the features of Countstar BioTech, Counstar BioMed provides the new characters as follow:

1.EPC-based Information System, storing the operation records all the time;

2. 5x magnification with wider test range: Cell diameter: 2-180μm；

3. Central data bank allows the user to keep all the data, like instrument test report, cell sample report and tester e-signature, safe and permanent.

4. level access user management system

5.EPC-based Information System, storing the operation records all the time;

6. The database supports sustainable management, and data can be traced through the sample No., operator and cell parameters;

Specifications

Technical Specifications

Model：

IM 1200

Test Item：

Concentration, Viability, Diameter, aggregation etc.

Sample Density：

1x104 - 3x107/ml

Sample Diameter：

2-180μm

Imaging element:

5 Megapixel，CMOS camera

Objective magnification:

5 X

Sample Volume：

20μL

Test Time：

<20s

Output：

JPEG/PDF/EXCE

Applications

Immune Cell Analysis

Countstar® BioFerm

Automated Yeast Cell Counter

Yeast Cell Count

Pitching Yeast

Brewing Yeast

Product Overview

The Countstar® BioFerm Automatic Yeast Counter combines traditional Melanie or Methylene Violet staining with high-resolution imaging and intelligent image recognition for accurate and consistent detection of yeast concentration and viability with a powerful Data Management System.

Key Benefits

Fast and easy operation, 20s for each sample

Dilution Free( 5x104 - 3x107 cells/ml )

Safe handling and disposal of samples with traditional stains such as Methylene Blue

Data easily comparable with a hemocytometer

Unique “Fixed Focus” image analysis provides reproducible data

Low cost and waste per test using disposable, each chamber slide with 5 chambers

Maintenance free

Product feature

Product Performance Testing

Diameter Measurement Comparison

Reproducibility test (25 samples, two staining methods for comparison with the hemocytometer)

Specifications

Technical Specifications

Model：

IY 1200

Test Item：

Concentration, Viability, Diameter, aggregation etc.

Sample Density：

1x104 - 3x107/ml

Sample Diameter：

2-180μm

Imaging element:

5 Megapixel，CMOS camera

Objective magnification:

5 X

Sample Volume：

20μL

Test Time：

<20s

Output：

JPEG/PDF/EXCE

Applications

The Countstar® BioFerm is able to accurately count the yeast cell with diameter between 2 to 180μm.

Example 1: Live Yeast Cell

Countstar® BioMarine Automatic Algae Counter

Algae Counting and Algae analysis

Biomass of Algae

Screening Target Algae

Product Overview

Integrating advanced optical imaging technology and intelligent image recognition technology, Countstar® BioMarine Automated Counter is a professional automatic counter developed to accurately measure the concentrations of algae.

For both pure cultures of unicellular algae and mixed cultures of multi-algae samples, Countstar® BioMarine can provide accurate results with high repeatability and save your valuable time and energy.

Key Benefits

Easy operation

20 sec. analysis time

Analyze different shapes of Algae

Wide Range Compatibility

Convenient screening

Product feature

Comprehensive algae information:

Countstar® BioMarine can automatically calculate the concentration, major axis length and minor axis length of algae and generate the algae growth curve, reflecting the growth of the algae.

Wide range compatibility:

Countstar® BioMarine is applicable to different shapes (i.e., spherical, elliptic, tubular, filamentous and cateniform) of unicellular and multi-cellular algae with axis length ranging from 2 µm to 180µm.

Convenient screening:

Countstar® BioMarine can screen target algae from complex samples for classification and counting by sizes or shapes.

Biomass Calculation:

To know the biomass of algae is basic for algae research. The traditional methods for analyzing biomass are Determination of the content of chlorophyll A – Accurate but complicated and time-consuming procedure. Spectrophotography – Need to use supersonic to destroy algae, not stable result and time-consuming.

Accurate and Excellent Reproducibility

Compared with the traditional hemocytometer, the result acquired by Countstar BioMarine shows a better linearity and a wider concentration range, and is thus more accurate.

Standard deviation shows Countstar BioMarine can accurately count selenastrum bibraianum cells; the error bar shows the average standard deviation.

Specifications

Technical Specifications

Model：

IA 1000

Test Item：

Concentration, Viability, Diameter, aggregation etc.

Sample Density：

1x104 - 3x107/ml

Sample Diameter：

2-180μm

Imaging element:

5 Megapixel，CMOS camera

Objective magnification:

5 X

Sample Volume：

20μL

Test Time：

<20s

Output：

JPEG/PDF/EXCE