#capsule staining

Negative staining: Principle, Procedure and Result

Negative staining procedure is used to study cell shape, cell breakage, capsule, surface inclusions and spores beside poly-beta-hydroxybutyrate granules. 

For bright field microscopy, negative staining is typically performed using a black ink fluid such as nigrosin and India ink. These are the acid stains. 

Due to the negative charged chromogen and negative charge on the cell wall of bacteria,…