#brain slices

Mouse brain

Luis de la Torre-Ubieta, Geschwind Laboratory, UCLA

However, you can see my Tumblr is very old-fashioned, just heaps of sentences. To be honest, I am not the visual kind of person. If you check out my Tumblr web page, i.e. not via app. (I seriously doubt whether people still are on Tumblr page.) My web theme really fits in my writings by the way.

Sometimes I wonder who would bother to read paragraphs after paragraphs on Tumblr. This is not a fan fiction Tumblr, after all. Then what’s the point of this Tumblr? OK, I just want to speak for my mind, mostly serious stuff but not sermons (it starts to sound like one.) 

People die but posts on the Internet last forever. Nothing is new under the sun, I believe some people think what I think, feel what I feel and I just happen to post it here. I write down ideas worth sharing here. If you are still reading this, big thanks to you. You are more admirable than you are. 

Have a good and a good life!

Brain slices protocol:  a complete protocol for obtaining in vitro and in vivo zebrafish brain preparations

“.....Recently, the zebrafish (Danio rerio) has been established as a key animal model in neuroscience. 

Behavioral, 

genetic, and 

immunohistochemical techniques 

have been used to describe the connectivity of diverse neural circuits. However, few studies have used zebrafish to understand the function of cerebral structures or to study neural circuits. 

Information about the techniques used to obtain a workable preparation is not readily available. 

Here, we describe a complete protocol for obtaining in vitro and in vivo zebrafish brain preparations. 

In addition, we performed extracellular recordings in the whole brain, brain slices, and immobilized nonanesthetized larval zebrafish to evaluate the viability of the tissue. 

Each type of preparation can be used to detect spontaneous activity, to determine patterns of activity in specific brain areas with unknown functions, or to assess the functional roles of different neuronal groups during brain development in zebrafish. 

Researchers Enlarge Brain Samples, Making Them Easier to Image

Read the full article Researchers Enlarge Brain Samples, Making Them Easier to Image at NeuroscienceNews.com.

New technique enables nanoscale-resolution microscopy of large biological specimens.

The research is in Science. (full access paywall)

Research: "Expansion microscopy" by Fei Chen, Paul W. Tillberg, and Edward S. Boyden in Science doi:10.1126/science.1260088 (http://dx.doi.org/10.1126/science.1260088)