Cutrale et al. describe in the latest Nature Methods issue, sophisticated new imaging processing software (Hyper-Spectral Phasors (HySP) ) that integrates 4 different staining tags plus the temporal information, allowing for an usnseen resolution for in vivo imaging.
You can find the original paper published in Nature Methods here.
Here is a quick glimpse at the highlights of the paper’s abstract and concluding marks:
“[..]” Time-lapse imaging of multiple labels is challenging for biological imaging as noise, photobleaching and phototoxicity compromise signal quality, while throughput can be limited by processing time. Here, we report software called Hyper-Spectral Phasors (HySP) for denoising and unmixing multiple spectrally overlapping fluorophores in a low signal-to-noise regime with fast analysis. We show that HySP enables unmixing of seven signals in time-lapse imaging of living zebrafish embryos.
Once more, zebrafish transparency comes out pretty handy!
