Mar 25, 2017
lmao these are kinda old but Hey heres that thing i was talking about before
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lmao these are kinda old but Hey heres that thing i was talking about before
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Another chemical got on my list: Aflatoxin B1, one of the most toxic chemicals that I’ve worked with.
Aflatoxin B1 is an aflatoxin produced by Aspergillus flavus and A. parasiticus. It is one of the most potent carcinogen known.
In animals, aflatoxin B1 has also been shown to be mutagenic, teratogenic, and to cause immunosuppression. According to the Food and Agriculture Organization (FAO), the worldwide maximum tolerated levels of aflatoxin B1 was reported to be in the range of 1–20 µg/kg in food, and 5–50 µg/kg in dietary cattle feed in 2003.
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I’m neglecting so much homework...
🌾 Food Safety Alert: Detect Aflatoxin with Confidence!
GeneMedi delivers complete aflatoxin rapid detection solutions – from raw antigens/antibodies to uncut sheets and ready-to-use test kits.
Ultra-sensitive detection levels: 🧪 Aflatoxin B1 (B1) ELISA IC₅₀: 0.02 ppb | LFT Cut-off: 0.5 ppb 🧪 Aflatoxin M1 (M1) ELISA IC₅₀: 0.02 ppb | LFT Cut-off: 0.2 ppb 🧪 Total Aflatoxin (AT) ELISA IC₅₀: 0.05 ppb | LFT Cut-off: 5 ppb These low detection limits ensure early warning and regulatory compliance in dairy, grains, nuts, and feed.
flexible supply from R&D to mass production! Need samples, pricing, or technical support for your aflatoxin assays? DM me or email [email protected]
Pakistan is an agricultural country and agriculture sector contributes approximately 35-40% to the national income. The Punjab is leading in the production of wheat. Wheat is more susceptible to infestation of different kinds of Fungi. Mycotoxins are perilous toxic metabolites fabricated abundantly by such Fungi. For a prolonged period of time, mycotoxins are familiar to attribute grievous diseases in mankind and animals. For this purpose, wheat flour was purchased from local markets and flour mills of different areas in the Punjab. A total of 108 whole wheat flour samples were collected and analyzed (73 samples from markets and 35 samples from flour mills) for aflatoxin, ash and moisture contents. The overall moisture of wheat flour sampled collected from flour mills of Punjab were in the range of 8.315% to 13.937% and from markets the values were in the range 10.302% to 13.803%.The overall ash content of wheat flour samples from flour mills were in the range of 0.618% to 0.983% and for market samples the values were 0.547% to 1.100%. All of the samples were analyzed and found negative for aflatoxin level in whole wheat flour. Results indicated that moisture content (%) in entire the flour samples were below 14%. Investigation of aflatoxin revealed that AFs (AFB1, AFG2, AFB2 and AFG1) were not established within detectable limits of Thin Layer Chromatography i.e. for B1 and G1 detectable limit is ≥0.72ppb while for B2, G2 is ≥0.20 ppb. However, aflatoxin was not be detected by thin layer chromatography, so for more reliable results, sophisticated techniques such as HPLC need to be used to determine aflatoxin in wheat flour samples.
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Aflatoxins are carcinogenic compounds produced by fungi like Aspergillus, which contaminate key crops such as maize, leading to food insecurity, poor health, and economic challenges, particularly in African countries where maize is a staple. High temperature, humidity, and insect damage increase aflatoxin contamination. Efforts to manage this issue are underway, with the most promising solution being the development of maize genotypes that accumulate lower levels of toxins and resist fungal infection. The present study was carried out to determine the reaction of maize genotypes to aflatoxin contamination in twenty selected exotic and local maize genotypes. The experiment was conducted at TARI-Ilonga in a screen house, and it was performed in accordance with a completely randomized design, with each germplasm replicated three times. The plants were inoculated with an A. flavus conidial suspension using the needle inoculation technique. An ultra-performance liquid chromatography fluorescence detector was used to quantify aflatoxin levels in the seeds of the maize genotypes. The results revealed varying degrees of aflatoxin contamination across the maize genotypes tested, ranging from 1.6 to 770.1 μg/kg. This study identified nine genotypes (G3, G10, G11, G12, G14, G15, G17, G18, and G20) that had very low aflatoxin concentrations (1.6–9.5 μg/kg). The identified genotypes can be tested in various environments and proposed for release as varieties or for use as parental materials in breeding programs to enhance food security, improve farmer incomes, and promote good health and sustainable agricultural practices in maize-growing regions.
Abstract The production of aflatoxin, a well-known mycotoxin, is primarily attributed to two distinct Emericella species and various Aspergi
Abstract The production of aflatoxin, a well-known mycotoxin, is primarily attributed to two distinct Emericella species and various Aspergillus species. Because of these mycotoxin high acute toxicity, immunosuppressive, mutagenic, teratogenic, and carcinogenic properties, aflatoxin poisoning has resulted in significant financial losses in the production of corn, cottonseed, groundnuts, sorghum, wheat, rice, and other commodities. Accordingly, the proposed study aims to characterize aflatoxin producing strains from various groundnut samples using morphological and molecular methods. A total of 11 isolates were found from an infected groundnut seed sample; qualitative analysis revealed that six of these samples were aflatoxin producing Aspergillus strains. Nevertheless, four samples were identified as producing aflatoxin by molecular detection using a gene specific primer involved in aflatoxin biosynthesis. These samples were collected from the districts of Dhule, Ahmednagar, Dapoli, and Latur in the Indian state of Maharashtra. The molecular method confirmed the highest level of accuracy when compared to other methods of detection; therefore, precise and unambiguous identification of aflatoxin producing Aspergillus spp. will be crucial for the development of control strategies for the fungus in groundnuts and groundnut products that are extensively consumed in India.
Aflatoxins are carcinogenic compounds produced by fungi like Aspergillus, which contaminate key crops such as maize, leading to food insecurity, poor health, and economic challenges, particularly in African countries where maize is a staple. High temperature, humidity, and insect damage increase aflatoxin contamination. Efforts to manage this issue are underway, with the most promising solution being the development of maize genotypes that accumulate lower levels of toxins and resist fungal infection. The present study was carried out to determine the reaction of maize genotypes to aflatoxin contamination in twenty selected exotic and local maize genotypes. The experiment was conducted at TARI-Ilonga in a screen house, and it was performed in accordance with a completely randomized design, with each germplasm replicated three times. The plants were inoculated with an A. flavus conidial suspension using the needle inoculation technique. An ultra-performance liquid chromatography fluorescence detector was used to quantify aflatoxin levels in the seeds of the maize genotypes. The results revealed varying degrees of aflatoxin contamination across the maize genotypes tested, ranging from 1.6 to 770.1 μg/kg. This study identified nine genotypes (G3, G10, G11, G12, G14, G15, G17, G18, and G20) that had very low aflatoxin concentrations (1.6–9.5 μg/kg). The identified genotypes can be tested in various environments and proposed for release as varieties or for use as parental materials in breeding programs to enhance food security, improve farmer incomes, and promote good health and sustainable agricultural practices in maize-growing regions.