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This study observed the yield response to nitrogenous fertilizer for a popular Boro rice variety BRRI dhan29 under silty loam texture soil with satisfactory organic matter (2.96%). Sequential elevated doses of nitrogen (N0, N70, N80, N90, N100, and N110 kg ha-1) along with recommended phosphorus (P), potassium (K), and Sulphur (S) significantly increased grain yield up to 50%. Tested rice variety yielded the highest from N110 kg ha-1 treated plot, 6.30-ton grain ha-1 and 7.57-ton straw ha-1, which were statistically similar with the 100 kg ha-1 nitrogen application. The N uptake from soil by the plant was also statistically equivalent for N100 and N110 kg ha-1 application. Panicle m-2, filled grain per panicle, and 1000-grain weight showed the same trends as N uptake and yield. The overall results demonstrate that the application of 100 kg N ha-1 was the best treatment for obtaining higher yield and the N content for both grain and straw of BRRI dhan29 at Old Brahmaputra Floodplain Soil.