Kalenial's Science Talk @kalenial-sciencetalk - Tumblr Blog

Ok so now I got a pylori smoothie what do I do with it ?

Well the smoothie is a mixture cell and flagella. I don’t want the cells so I do a low speed spin in centrifuge to pellet cells and leaves flagella in solutions. Next I take the flagella solution and do a high speed spin to pellet all the flagella.

Next step (which might change in the future) i purify it though a sucrose gradient which has layers of different concentration of sucrose.

Each color is a different layer of sucrose. Sadly not usually this colorful. My student added food coloring to make the layers more visible for a picture for her presentation.

I add flagella pellet which has been dissolve in a buffer on the top and do another high speed spin overnight

The next day all the flagella will have move down to a lawyer of sucrose that has the same density as it. So that cloudy band should just be all my flagella and only flagella.

#science #bacteria #microbiology #phd research #sillydor

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It smoothie day!

Pylori smoothie lol

So all the cells I grew yesterday are now in the blender. I’m taking off their flagella with blender. Now they can’t swim mwahahahahah

If they can’t swim they can’t infect lol

#flagella prep #science #sillydor #phd research

Pretty science picture time !

So I’m feeling motivated this week in lab again.

For those who don’t know, my main project in lab is looking to see if this efflux pump in pylori is involved in making flagella. Im order to check this it involves lots of flagella samples lol. Which leads me to my sometimes weekly 40 plates of bacteria

Behold my army !

This is actually 39 plates lol, since one of them got contamination.

#science #flagella #hpylori #phd research #sillydor

mirrorballsworld

According to UIS data, less than 30% of the world’s researchers are women. UIS data also show the extent to which these women work in the public, private or academic sectors, as well as their fields of research. But to truly reduce the gender gap, we must go beyond the hard numbers and identify the qualitative factors that deter women from pursuing careers in science, technology, engineering and mathematics (STEM).

Women in STEM are capable of anything and are an encouragement for many young girls. Rosalind Franklin, Martha Chase and Barbara McClintock (to name a few) changed the world with their work and now Emmanuelle Charpentier and Jennifer Doudna are paying new roads and inspiring millions.

#science #women in stem

Okay so I don’t know if I ever talked about the organism I work on in lab.

My model organism is Helicobacter pylori

A gram negative bacterium that is usually found in the human stomach. It has a helical shape and typically has 4-6 flagella found on one of the poles.

Looks kind of like this lol. The light blue rod. Small gif that I made for fun.

So weird thing about this organism is most H. pylori infections aren’t symptomatic. We don’t really know why some people exhibit symptoms and others don’t. People with symptoms usually get stomache ulcers (gastritis) and in a small percentage of patients they end up with chronic gastritis that develope into certain cancers. Which makes pylori the only bacteria to be currently classified as a carcinogen.

#bacteria #h.pylori #science #myart #microbiology

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inchoatewaffle

Angry not mad Scientist

I think we need more angry scientist blogs. Cause there is a lot to be angry about in the world today. also kudos to anyone who got the ref I made in the title lol.

lycanthology

i just said the same thing on twitter but please please please consider standing up for remote learning options even if everything does somehow go back to normal. so many physically disabled people drop out of school/college because of inaccessibilty. we have been asking for an option like this for so long and keeping it would change the lives of sooooo many people. we have an opportunity to start reforming the educational system to be more accessible to disabled and poor people and i really dont want to lose remote options because ableds are too lazy to keep hosting video calls.

this is okay to reblog but physically abled ppl be quiet!

#remote learning

When you have left over dry ice from freezing your bacteria culture. Look at dat smoke !

For those who have not worked in microbio lab, for long term storage of our bacteria strains we freeze them and store them at -80 C freezer. This keeps them viable for future use. We do this instead of just keeping them in fridge because they can still grow in the fridge just very slowly. And when the cells grow they get old which makes them not grow very well. Additionally, since bacteria can grow quickly they have a tendency to accumulate mutations which can be problematic if you are trying to study a specific gene in the bacteria. Therefore we keep them frozen til we need them.

Dry ice is a way to freeze the bacteria cultures really quick and a way to keep them frozen when I need to take a bit of icy frozen bacteria to grow more bacteria on a plate.

The picture is the result of adding water to my dry ice alcohol mixture lol. Frozen waste land.

#science #dry ice #lab

positively--speculative

The reason Black women in fandom get so frustrated with white women in fandom (especially the ones who claim they are feminist) is that white women in fandom have decided what is good for Black women despite Black women arguing otherwise.

White women will see Black characters like Valkyrie/Brunhilde and strip her of her sexuality and decide she “doesn’t need a love interest” and suggest shipping her with Thor strips her of her power. Either this or they will claim shipping her with Thor erases her bisexuality when the meaning of bisexuality is to be attracted to more than one gender (and she already had a girlfriend, but you don’t see them fighting to have future movies explore that). White women will treat news that Ariel will be played by a Black young adult and say “little Black girls deserve better” than to see themselves as vulnerable and demure. The problem with this mindset is it neglects to acknowledge that Black women have *always* been seen as “strong and independent.” It is nothing new to us.

At the same time, white women in fandom will see themselves in a character like Rey–a woman who started off as all of the things they claimed they wanted to see in a female character: strong, independent, and likeable–and reduce her by giving her the job of *fixing* an overpowered and dangerous white villain all to keep her away from the Black character

azarathedragon

Yea. Aleays remember that the stuff one woman wants is not inherently the same as other women.

Moana for example. No love interest and suffers losses. Strong and independent, ultimately successful in her story. But shes a WoC. And the successful part is awesome. But the rest isnt. Because thats what WoC have always been fed. Never the flowery princessy girls. Never the swooning damsel being rescued. (And the times where they do get a girl like that, she gets killed off. *cough cough* Allura from VLD).

Meanwhile. Rapunzel from Tangled gets: a love interest genuinely invested in her, still strong and grows to be independent, still meets trafitional female tropes of being girly and doing girly things, a successful ending to her story where only the bad guys die. No long term suffering.

“I dont need no man” does not equal “I dont want a man.” Strong independence does not and should not mean abandoning traditional female tropes and values. Let our ladies out there (regardless of color) still be cutesy, love gardening and baking and sewing and whatever else. Let them find fufilling love interests that respect them. Stop murdering them for shitty low tier drama (Allura. Missandei. And so. Many. More.). Stop handing them off to the first shitty man who sits around and waits for her, never bothering to get to know her or do things she enjoys and actually make an effort to be attractive. Just. Fucking talk to the real people this character draws off of. What do they want to see in the characters that represent them?

serotonesque

I’ve seen this picture going viral without attribution.

This picture was taken in Columbia, SC, May 31st by local photojournalist Crush Rush.

Crush has an amazing and powerful portfolio! Please check it out:

On his Website

On his Instagram

On his Blog

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programmerhumour

Funny how that works

ferrousferrule

I am so pleased at how many notes are some version of “I don’t fear the science, I fear the corporations who control it” because that is EXACTLY the attitude you should have. GMOs can save us. Monsanto will kill us.

brunhiddensmusings

what people fear about GMO- ‘theyre gonna make frankencarrots that crave human flesh and cause diarrhea ’ what GMO actually is- ‘we made rice crop that is both drought resistant and flood resistant which will prevent about 20% of major famine disasters, also it now makes vitamin A because vitamin A deficiency in poverty stricken areas is a major killer of kids as most vitamin A rich foods dont grow there’ what people SHOULD be upset about- ‘i made all crops sterile so all farmers have to buy the seed from me in perpetuity and i will sue anyone who tries to go back to crops that produce their own seed’

aniseandspearmint

^^^ THIS

#gmo #science

Wanted to share some cute water color microbes I painted for my students last year

#biology #bacteria #my art

Does anyone even check this blog? I was hoping to actually start science talks but I haven’t figured out a way I would like to run it. So until then I was thinking of putting up my current research stuff because I have a lot of random pictures from my lab.

#anyone here ?#science #sillydor

gardenforsparrows

For all you students who are suddenly finding yourself with online classes, here is list of graduate students who are willing to help out with anyone struggling and our specialties/areas of interests. We’re all colleagues and we’re all in this together! Let’s be a true academic community and support each other as much as possible in the coming months.

ceebycee - introductory physics, modern physics, quantum mechanics, astronomy, calculus (differential, integral, multivariable), linear algebra

@feamir - upper level physics and math

@andbeavillain - anything science and math related up to the university level, editing papers of any subject for clarity and grammar

@softcroft - biochemistry, biology (particularly genomics and transcriptomics), public health, epidemiology, social and behavioural health, PH biostats, organic chemistry, scientific writing, presentations, resume/CV/interview prep for bio sciences, Latin language

@cancerbiophd - biology (human physiology, mammalian cells, biology lab techniques, cancer), fellowship applications, grant writing, presentations, academic/scientific communication

irishfencer456 - biology (genetics, cell biology, signal transduction, intro biology subjects), technical writing, fellowship applications, grant writing, presentations, academic/scientific communication

@studyevergreen - environmental studies, biology, lower level chemistry, physiology, toxicology, environmental education, environmental governance, high school level subjects, proofreading and editing

@adelicateculturecell - general biology, genetics, basic chemistry

@xmarksthenerd - general biology, molecular biology, some orgo and biochemistry, algebra and geometry

thesleepiestsleepscientist - biochemistry, systems neuroscience, human physiology

@my-lions-mane - anatomy, human biology/anatomy, developmental biology, biochemistry, forensic toxicology, microbiology, interviews, applications, and statments

@narrativerehearsal - osteoarchaeology, archaeological science (isotopes, radiocarbon dating), skeletal anatomy and pathology, general archaeology and world prehistory, some medieval history (mainly British), some zooarchaeology

caffeinated-phd - computer science, Java, Scala, Python, C, C++, C#, Haskell, HTML, CSS, JavaScript

@professorerudite - English and Japanese

@myskyisempty - psychology, educational psychology, counseling and education

@gardenforsparrows - outdoor education/recreation, environmental education, environmental science, program evaluation, GIS, lower level english and literature

And speaking for myself, I’m more than happy to chat or answer questions about what I’m studying for anyone who’s curious, regardless of whether or not your studying it!

It you want to be on (or off) this list, just send me a message.

queenofthebench

SDS-PAGE Hall of Shame

I found this hilarious website from Rice University and had to share!

The comments are so wonderful. I am currently laughing out loud at my desk xD

https://www.ruf.rice.edu/~bioslabs/studies/sds-page/sdsgoofs.html

jobenhoben-deactivated20250517

I bet some of these are still publication-grade!

#science #gels

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studyquill

Notetaking

Sound Note - take notes while you record audio

Evernote - notetaking that syncs across platforms

Paper 53 - minimal notetaking that syncs

Microsoft OneNote - collaboration and syncing, best for Office users

Google Keep - jot things down, best for Google suite users

Notability - take notes and annotate PDFs

Mindly - create mind maps

Day One - a digital journal

Flash Cards

Quizlet - the quintessential flash card app

StudyBlue - another commonly used app

Cram - best for its “cram mode”

Eidetic - uses spaced repetition for effective memorization

Planner

My Study Life - schedules, tasks, reminders, and more

StudyCal - keeps track of tasks, exams, and grades

24me - automated reminders and event planning

iStudiez - schedule and prioritized task list

Google Calendar - a calendar, best for Google users

Glass Planner - a calendar and to do list with incredible functionality

To Do List

Clear - organized to-do and reminders

MinimaList - simple to-do and focus timer

Trello - collaborative project organizer

Todoist - clean and functional task manager

Default notes app on your phone

Time Management

Forest - plant trees by staying focused

Pomotodo - pomodoro timer with to-do list

Timeglass - custom timers

Tide - pomodoro with white noise

Alarmy - forces you out of bed

Pillow - smart alarm that tracks sleep cycles

Productivity

Workflow - automate tasks

Habitica - turn your habits into an RPG

Continuo - simple, colorful activity tracking

Freedom - block distracting apps

Free Learning

Coursera - free MOOCs

TED - listen to Ted Talks

Duolingo - language learning

Memrise - spaced repetition language vocabulary

Khan Academy - free video lessons

Ambient Noise

8tracks - curated playlists

Spotify - online music streaming

Coffitivity - cafe ambience

Noisli - background sound generator

Rain Rain - rain sounds

Binaural - binaural beats

Health

Rockin Ramen - recipes based on ramen

MealBoard - meal planning

Lifesum - healthy eating

Stop Breath And Think - mindfulness meditation

Pacifica - mental health management

Sworkit - personalized video workouts

Waterlogged - hydration tracker

Reference

WolframAlpha - Google on steroids

Oxford Dictionary - all of English at your fingertips

RefMe - citation generator

PhotoMath - solve math problems by taking a photo

Mathway - step by step math help

Desmos - free graphing calculator

Wikipedia - not the best source, but it’s handy

Miscellaneous

Companion - stay safe when walking alone

Mint - money management

Toshl - finance manager

Tiny Scanner - scan documents

cancerbiophd

Relax Melodies is my favorite Ambient Noise app (available on Google and Apple App stores) - you get to mix and match a bunch of sounds, everything from snow falling on rooftops to campfires to cats purring.

#school stuff

amoebasisters

NEW video on microscopes!! Huge thanks to our patrons for the topic selection and support!

#school stuff

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