(^ from @eridan-ampora's transcript)

I figured that there was some dialogue we missed, so I went and looked through the transcript to see if anything else jumped out at me:

(i didn't go any further than this because this is where we stopped playing and i don't want to spoil bee on the rest of the game)

i feel that there are a lot of small aspects of xefros' character in these games that could support him being transfem. most, if not all of these aspects can be mainly attributed to other factors (such as his place on the hemospectrum, or the way dammek treats him), but i find that these things can go hand-in-hand with a trans reading (like how, for example, june's sense of being adrift and disconnection from the world around her is a symptom of how she clings to the false ideal of her childhood, but it's also due to her living pre-transition as an inauthentic shell of herself)

one big aspect is that he's consistently self-depricating and has little to no self-confidence—a distinct symptom of dysphoria

something that i think is interesting is that this is an intentional subversion of the jock trope that he embodies, but it wouldn't make sense for the game's secondary protagonist to stay this way forever without any personal growth. homestuck's overall story places heavy emphasis on subverting norms, so being transfem would continue to follow this theme

going back to that fourth screenshot, i remembered that the song that plays in xefros' hive is this one:

that is, it plays in every room of his house... except for his sports room, the 1 room where he allows himself to have fun, be a kid, and overall be a more authentic version of himself. so, given the context of how it's used in act 1 and 2, "keeping your head down" represents repressing that authentic part of yourself in response to authoritative force and danger

another aspect is that xefros is someone who can be heavily in denial of what's going on:

and sees himself dying young:

these aspects could be reasons for xefros surpressing a potential trans realization

it even adds a new layer of depth to his moirallegiance with dammek; i've made comparisons before between them and cases of men who've fought for workers' rights and class equality who then leave their wives to cook, clean, and do the dishes:

so this is further supported with the possibility of xefros being a girl