#zc45

Abstract

SARS coronavirus (SARS-CoV), the causative agent of the large SARS outbreak in 2003, originated in bats. Many SARS-like coronaviruses (SL-CoVs) have been detected in bats, particularly those that reside in China, Europe, and Africa. To further understand the evolutionary relationship between SARS-CoV and its reservoirs, 334 bats were collected from Zhoushan city, Zhejiang province, China, between 2015 and 2017. PCR amplification of the conserved coronaviral protein RdRp detected coronaviruses in 26.65% of bats belonging to this region, and this number was influenced by seasonal changes. Full genomic analyses of the two new SL-CoVs from Zhoushan (ZXC21 and ZC45) showed that their genomes were 29,732 nucleotides (nt) and 29,802 nt in length, respectively, with 13 open reading frames (ORFs). These results revealed 81% shared nucleotide identity with human/civet SARS CoVs, which was more distant than that observed previously for bat SL-CoVs in China. Importantly, using pathogenic tests, we found that the virus can reproduce and cause disease in suckling rats, and further studies showed that the virus-like particles can be observed in the brains of suckling rats by electron microscopy. Thus, this study increased our understanding of the genetic diversity of the SL-CoVs carried by bats and also provided a new perspective to study the possibility of cross-species transmission of SL-CoVs using suckling rats as an animal model.

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“SARS-CoV-2 has a telltale signature of genetic engineering, not previously identified . That genetic fingerprint suggests the work of Ralph Baric, Ph.D., was used in the creation of the virus. There’s a direct match between Baric’s published research — which describes how to hide telltale signs of genetic engineering — and the genetics found in SARS-CoV-2

In 2002, Baric invented a technique called seamless ligation, which conceals all evidence of genetic engineering in lab-created pathogens. Baric’s nickname for this technique is the “no-see’m method.” Baric taught the method to Shi Zhengli in 2016, and Shi and her colleagues at the Wuhan Institute of Virology (WIV) demonstrated mastery of Baric’s technique in a series of gain-of-function experiments. However, while seamless ligation conceals human tampering in lab-created pathogens, the method leaves a signature of its own, and that’s the signature discovered in SARS-CoV-2.

The findings raise the possibility of liability for the University of North Carolina where Baric works, the National Institutes of Allergy and Infectious Diseases, which funded Baric, the WIV and other parties”

H/T

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April 2020:

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Researchers say one in 3 trillion chance Covid developed the code naturally

https://www.frontiersin.org/articles/10.3389/fviro.2022.834808/full#B10

https://www.ncbi.nlm.nih.gov/labs/pmc/articles/PMC7457603/

Abstract

SARS coronavirus (SARS-CoV), the causative agent of the large SARS outbreak in 2003, originated in bats. Many SARS-like coronaviruses (SL-CoVs) have been detected in bats, particularly those that reside in China, Europe, and Africa. To further understand the evolutionary relationship between SARS-CoV and its reservoirs, 334 bats were collected from Zhoushan city, Zhejiang province, China, between 2015 and 2017. PCR amplification of the conserved coronaviral protein RdRp detected coronaviruses in 26.65% of bats belonging to this region, and this number was influenced by seasonal changes. Full genomic analyses of the two new SL-CoVs from Zhoushan (ZXC21 and ZC45) showed that their genomes were 29,732 nucleotides (nt) and 29,802 nt in length, respectively, with 13 open reading frames (ORFs). These results revealed 81% shared nucleotide identity with human/civet SARS CoVs, which was more distant than that observed previously for bat SL-CoVs in China. Importantly, using pathogenic tests, we found that the virus can reproduce and cause disease in suckling rats, and further studies showed that the virus-like particles can be observed in the brains of suckling rats by electron microscopy. Thus, this study increased our understanding of the genetic diversity of the SL-CoVs carried by bats and also provided a new perspective to study the possibility of cross-species transmission of SL-CoVs using suckling rats as an animal model.

1Department of Epidemiology, College of Preventive Medicine, Third Military Medical University, Chongqing, 400038 China

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