Wheat (Triticum aestivum L.) seeds (100 accessions) were selected on the basis of different geographical areas of Pakistan. Isolation and identification of seed born fungi were conducted according to standard blotter test. A total of five major seed borne fungi including Alternaria alternata, Aspergillus niger, Fusarium sp., Drechslera sp. and Phytophthora sp. were isolated from the wheat seeds. The frequency ofoccurrence of these five seed born fungi Alternaria alternata, Aspergillus niger, Fusarium sp. Drechslera sp. and Phytophthora sp. was 49%, 46%, 42%, 35% and 16%, respectively. Infection percentage varied from 0-90% in all 100 wheat accessions. Among the accessions, highest infection (100%) of seed born fungi was recorded in 011185 and 011757 while lowest infection (10%) was recorded in 011415 accessions. Moreover,in accessions collected from Gilgit Baltistan and AJK, Alternaria niger and Alternaria fusarium were dominant, while in Kpk province, Aspergillous niger was prevalent followed by Alternaria alternata. In case of Baluchistan province, the dominant seed born fungi was Alternaria alternata followed by Drechslera spp. Similarly in case of Punjab, the occurrence of Alternaria alternata, Drechslera sp , Fusariumsp, and Aspergillous niger associated with seeds were similar. For accession collected from Sindh province, the dominant seed born fungi was Aspergillous niger and Drechslera sp. However, the Phytophthora sp. infection of wheat seeds accession of Baluchistan was highest followed bywheat seeds accession collected from Gilgit Baltistan and AJK, Kpk and Punjab whereas wheat seeds accessions collected from Sindh province were found to be free from Phytophthora species.
