Currently enzymatic detergents emerged as better option due to their cleaning and stain removal effectiveness in comparison to the synthetic surfactants. An extracellular alkaline protease produced by Bacillus subtilis strain FH1 was studied for its potential application as detergent additive. Enzyme was tested for its compatibility and stability by incubating it with several detergents like Surf Excel, Ariel, Express, Bonus and Brite and residual activity was checked by standard enzyme assay, it showed 92, 85, 100, 75 and 126% residual activity respectively. Its wash performance was checked by adding in these detergents while washing manually soiled cotton cloth pieces and showed excellent wash performance in combination with detergents at lower temperature. Due to stability and good wash performance the enzyme can be used as detergent additive.
