#transgenesis

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Transgenesis is described as the stable integration, expression, and transfer of exogenous DNA into the host genome using various means like as microinjection, sperm mediation, electroporation, and so on. The melanin concentrating hormone gene was used to perform sperm-mediated gene transfer in ROHU (Labeo rohita) in this investigation (p-CMV-MCH). Milt was utilised to fertilise the eggs after being bathed in various concentrations of plasmid DNA (50-200 g / ml) for 10 minutes. The incorporation of transgene was studied via slot blot analysis. It exhibited foreign gene integration, indicating that carp sperm can pick up DNA in around 10 minutes. Furthermore, the MCH gene causes melanophores in the body, caudal region, and on the eyeball to shrink, resulting in transgenic fish that are brilliant. As a result, our research clearly shows that the MCH gene construct can be employed as a transgenesis marker gene and to investigate the molecular basis of gene integration expression and transmission. Please see the link :- http://mbimph.com/index.php/UPJOZ/article/view/1755

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'Transgenesis' may be defined as the introduction of exogenous DNA by different methods such as microinjection, sperm mediation electroporation etc. into the host genome resulting in its stable integration, expression and transmission. In the present study sperm-mediated gene transfer was carried out in ROHU (Labeo rohita) using melanin concentrating hormone gene, (p-CMV-MCH). Milt was soaked in different concentrations of plasmid DNA (50-200 µg / ml) for 10 minutes and it was used to fertilize the eggs. Slot blot analysis was carried out to study the integration of transgene. It showed integration of foreign genes which indicates that the carp sperm do pick up DNA in about 10 minutes. Moreover the MCH gene causes contraction of melanophores in the body, caudal region and on the eyeball resulting in brightness of the transgenic fish. Therefore this study clearly indicates that MCH gene construct can be used as a marker gene to identify transgenesis and to study the molecular basis of gene integration expression and transmission. Please see the link :- https://mbimph.com/index.php/UPJOZ/article/view/1755

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Scientific experiments are the new rituals that require human sacrifice: we have never been modern 

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