The current study looked at how Listeria monocytogenes (ATCC 657) and Aeromonas hydrophila (ATCC 646) reacted to anti-listeria and anti-aeromonas compounds formed by various LAB (Lactic acid bacteria) such as fish bacteria. The presence of LAB in fish intestine was investigated in order to see whether it could be used in fish biopreservation. An agar well diffusion assay was used to evaluate the combative behaviour of ten LABs against pathogenic and spoilage flora. LAB isolates were effective in eradicating indicator strains using either live cells and CFS (cell-free supernatant) or live cells alone. Antimicrobials were developed to work against Gram positive and Gram negative bacteria. Despite the fact that various forms of antagonism have been identified. Also after heating at 90°C for 10 minutes, the antimicrobial activity of neutralised and catalase treated cell free supernatant fluid was maintained against Listeria. Thermostable goods had the most protection against Listeria. Antagonism was not found to be associated with any anti-aeromonas thermostable products. Aeromonas was resistant to just a few thermolabile materials. The majority of LAB-like isolates treated with thermostable anti-listeria products were found to be effective against Aeromonas with only cells. Proteinaceous antimicrobials that are effective against Aeromonas and Listeria found in two isolates may be more effective in fish preservation than bacteriocins that are primarily effective against Gram positive bacteria. In situ application of indigenous natural bacteriocins of Oreochromis niloticus [Linnaeus, 1758] for fish shelf life extension is advocated in the report. Please see the link :- https://mbimph.com/index.php/UPJOZ/article/view/2031

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