The present study is part of a vast program of valorization of the medicinal flora in order to help the populations to draw a real profit from the use of the plants. The objective of this work was to determine through a phytochemical screening, the main chemical groups and the evaluation of the estrogenic effect of the aqueous extract of the curled leaves of P. crispum in rats. To achieve this objective, a tri-phytochemistry was performed on the aqueous extract of P. crispum leaves and compounds such as phenols, flavonoids, catechic tannins, saponosides, sterols and polyterpenes were investigated. For the study of estrogenic properties, four groups of six rats were formed and treated orally. Group 1 received distilled water and constituted the control lot. Groups 2, 3 and 4 received the aqueous extract of P. crispum leaves at doses of 50, 100 and 200 mg/kg body weight, respectively. The results of this study revealed that the aqueous extract of P. crispum leaves maintained the estrous cycle in estrus and significantly increased ovarian mass and uterine horn. The concentration of reproductive hormones increases significantly at doses of 50 and 100 mg/kg body weight. At these doses, the extract also stimulates follicular development and ovulation, which are at the origin of the estrus phenomenon. Thus its traditional use in the treatment of infertility is justified.
