Top Posts Tagged with #ndei

Lack of political will, underfunding stall East-West road project

• N82b Needed For Accelerated Completion— Akpabio Lack of political will and gross underfunding have hindered the completion of the East-West Road started 14 years ago. Despite the economic importance of the road, which crisscrossed five out of the six states in the South-South, stakeholders in the region have continually decried the present administration’s indifference towards completion of the…

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#East-West road project #Goodluck Jonathan #NDEI #PESOA

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NdeI activity is sensitive to contaminants present in DNA isolated with standard miniprep protocols. Further purification of the DNA by a column or dialyzing the contaminant from the DNA may help increase the activity of the enzyme on the isolated DNA substrate.

https://www.neb.com/faqs/2011/11/10/it-seems-that-ndei-is-having-some-difficulties-cutting-my-dna-is-there-a-reason-for-that

And all of a sudden this might be why my construct is wrong. Remember kids, on a gel you can see the difference easily for bands <3kb, but more than 8kb and you’ve got to run that gel for a really long time. I’ll confirm soon that NdeI isn’t cutting, but this likely is why none of my constructs contain FUS.

#FUS #NdeI #gel electrophoresis #troubleshooting #NEB