#bioinformatics.

Cotton tolerance to high temperatures is a complicated feature governed by numerous genes that work together to avoid or withstand high temperatures. When exposed to high temperatures, all genotypes may display quantitative and qualitative changes in gene expression. Heat Shock Protein is involved in several stages of plant development, growth, and regulation of multiple pathways in higher plants that are involved in abiotic stress tolerance. In this study, special primers were constructed to isolate and identify certain genes responsible for high temperature tolerance in Gossypium barbadense. Hsp21, Hsp26, Hsp23, and Hsp36 are four heat shock protein (HSP) genes that have been isolated, sequenced, and identified. Database searches revealed that the four segments correspond to four genes with significant similarity in Gossypium sp. and are involved in heat treatment. Sequences in Multiples Alignment and comparison mapping revealed the link between sequences and function, elucidating the mechanism of adaptation that is genotype-dependent. Through genetic manipulation and molecular breeding, it is possible to improve cotton tolerance to high temperatures by understanding the tolerant mechanism and its gene activity.