#bacteriocin

Nosocomial infections due to methicillin-resistant Staphylococcus aureus (MRSA) are a major public health problem worldwide. The main aim of this study was to evaluate the inhibitory potential of lactic acid bacteria (LAB) against MRSA. Six strains of LAB isolated from camel milk, were identified by phenotypic method, which revealed their belonging to the species “Lc.lactis subsp.lactis”; “Lc.lactis subsp.lactis var diacetylactis”; “Lc. lactis subsp. cremoris”; “Enterococcus durans”; “Ln.mesenteroides subsp. mesenteroides”; “Lb. Plantarium”. The antimicrobial activity of these selected strains is examined against five (05) strains of methicillin-resistant Staphylococcus aureus (MRSA) isolated from patients with infections in the intensive care unit of Slimane Amirate hospital, in the wilaya of Touggourt (Algeria) and also against two (02) control strain: methicillin-sensitive Staphylococcus aureus MSSA 25923 ATCC and Methicillin-resistant Staphylococcus aureus MRSA 43300 ATCC. Then the treatment of the culture supernatants of the isolated strains, selected antagonists “Ln. mesenteroides subsp mesenteroides” and “Lb. Plantarum” with proteolytic enzymes inactivated their inhibitory effect, indicating that the agents responsible for the inhibitions are bacteriocins. The physico-chemical characterization of bacteriocins revealed their thermo-resistance and their stability at acidic and basic pH. The results presented in this study provide a clearer idea of the antibacterial potential of bacteriocins produced by Leuconostoc mesenteroides and Lactobacillus plantarum, which represent a way forward to compensate for antibiotic treatments.

This paper aims to examine the isolation of Lactobacillus from dairy products (milk, curd, and yogurt), extraction of Bacteriocin from it as well as to determine their inhibitory effect against few fungal skin pathogens and bacterial skin pathogens such as: Candida albicans, Aspergillus sp., Malassezia sp., Fusarium sp., and Penicillium sp., Staphylococcus aureus, Streptococcus pyogens, Klebsiella sp. The antagonistic activity of Lactobacillus sp. is mainly due to the bacteriocin present in it, therefore in this study, the bacteriocin is extracted and checked against the indicated microorganisms. Kirby Bauer Disc Diffussion method is used and zone of clearance is observed around the pathogenic species indicating that they shows some kind of antagonistic activities which are further measured and noted, which gives a clear picture about the degree of resistance of bacteriocin against the pathogenic microorganisms.

This paper aims to examine the isolation of Lactobacillus from dairy products (milk, curd, and yogurt), extraction of Bacteriocin from it as well as to determine their inhibitory effect against few fungal skin pathogens and bacterial skin pathogens such as: Candida albicans, Aspergillus sp., Malassezia sp., Fusarium sp., and Penicillium sp., Staphylococcus aureus, Streptococcus pyogens, Klebsiella sp. The antagonistic activity of Lactobacillus sp. is mainly due to the bacteriocin present in it, therefore in this study, the bacteriocin is extracted and checked against the indicated microorganisms. Kirby Bauer Disc Diffussion method is used and zone of clearance is observed around the pathogenic species indicating that they shows some kind of antagonistic activities which are further measured and noted, which gives a clear picture about the degree of resistance of bacteriocin against the pathogenic microorganisms.

This paper aims to examine the isolation of Lactobacillus from dairy products (milk, curd, and yogurt), extraction of Bacteriocin from it as well as to determine their inhibitory effect against few fungal skin pathogens and bacterial skin pathogens such as: Candida albicans, Aspergillus sp., Malassezia sp., Fusarium sp., and Penicillium sp., Staphylococcus aureus, Streptococcus pyogens, Klebsiella sp. The antagonistic activity of Lactobacillus sp. is mainly due to the bacteriocin present in it, therefore in this study, the bacteriocin is extracted and checked against the indicated microorganisms. Kirby Bauer Disc Diffussion method is used and zone of clearance is observed around the pathogenic species indicating that they shows some kind of antagonistic activities which are further measured and noted, which gives a clear picture about the degree of resistance of bacteriocin against the pathogenic microorganisms.

This paper aims to examine the isolation of Lactobacillus from dairy products (milk, curd, and yogurt), extraction of Bacteriocin from it as well as to determine their inhibitory effect against few fungal skin pathogens and bacterial skin pathogens such as: Candida albicans, Aspergillus sp., Malassezia sp., Fusarium sp., and Penicillium sp., Staphylococcus aureus, Streptococcus pyogens, Klebsiella sp. The antagonistic activity of Lactobacillus sp. is mainly due to the bacteriocin present in it, therefore in this study, the bacteriocin is extracted and checked against the indicated microorganisms. Kirby Bauer Disc Diffussion method is used and zone of clearance is observed around the pathogenic species indicating that they shows some kind of antagonistic activities which are further measured and noted, which gives a clear picture about the degree of resistance of bacteriocin against the pathogenic microorganisms.