#bacteriatesting

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Methods of evaluation

JIS L 1902 is similar to standard antimicrobial textile test methods that examine textile materials following a brief incubation period. Ideally, this method is used for high throughput, accelerated turnaround, and substantial volume testing.

Absorption Method

In this method, the test bacteria is inoculated directly onto the sample. The standard contact time for the JIS L 1902 test method is 18 to 24 hours. The number of bacteria present directly after inoculation and after the stipulated contact time is measured and used to estimate the antibacterial activity.

Transfer Method

This evaluation method consists of placing the test bacteria on an agar plate and subsequently transferring them onto the samples. The sample textile materials are kept in contact with the bacteria for five minutes and incubated for 18 to 24 hours. The number of bacteria present directly after inoculation and after the stipulated contact time is measured and used to estimate the antibacterial activity.

Also Read: The ISO 21702 Test Method Explained

Printing Method

In this evaluation method, the test bacteria are placed on a filter and printed onto the samples.

Halo Method

The textile sample is kept in contact with the surface of these inoculated agar plates. The plates are incubated for 24 to 48 hours and then assessed for “halos”. This is a qualitative method that estimates antibacterial activity through the appearance of halos [clear area of growth] around the sample.