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How are 5-ROX and 6-ROX dyes different from one another?
How are 5-ROX and 6-ROX dyes different from one another? The ROX dye is connected to the nucleic acid molecule at spots 5 and 6, which are shown by the “5” and “6” prefixes. The dyes’ chemical characteristics and practical uses are similar; however, the dyes may have different fluorescence qualities. 5-ROX: This dye, which is usually attached to the 5′ end of an oligonucleotide probe, gives off…
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