If you want to find out how your immune system reacts to a certain disease, an important first step is to identify the type of immune cells that are activated in that case. The immune system is incredibly complex, but it can be broken down into two types of T cells: T helper cells and T suppressor cells. If you want to learn more about these cell types and the diseases they can cause, then a foxp3 staining kit may be exactly what you need in your research laboratory! Read on to learn how this useful kit works and how it can help you carry out your next immunology experiment with ease!

What you get?

The Foxp3 staining kit includes everything you need to identify, quantify, and investigate Tregs in your studies. The included manual walks you through staining human and mouse samples, then transferring them onto a microscope slide for observation under different light wavelengths. When used correctly, it will allow you to visualize (and count) both CD4+ and CD8+ T cells that express FOXP3. You can even stain cryopreserved cells or tissue sections from stored formalin-fixed tissues!

How it works?

The Foxp3 staining kit contains everything you need to stain CD4+ CD25+ T regulatory cells (Tregs) and measure their frequency in peripheral blood samples. While a number of commercial antibodies exist for quantifying Tregs, staining for FOXP3 is preferred because it shows greater specificity, sensitivity, and accuracy. The kit contains all of the reagents needed to stain 20–50 sample wells.

Why use it?

There are many studies that try to analyze and characterize innate immune cells in order to see if they can be reprogrammed into Tregs. However, most of these studies require special flow cytometry methods in order to identify and quantify them (i.e., CD4+CD25+FoxP3). But not everyone has access to these kinds of high-end systems, so researchers who lack them are forced to look for workarounds.

The risks of not using it

When performing research using animal models, it’s common to need to assess whether or not certain immune cells are present in certain regions of a subject’s body. Most researchers take care of these tasks by hand, which can be time-consuming and difficult for graduate students and other lab members who might not have experience immunological techniques. Additionally, lack of proper training can lead to errors—and these mistakes could lead you to false conclusions or improper results.

How to get it?