Fluorescence spectroscopy
Fluorescence spectroscopy measures both the fluorescent properties and concentration of a substance, as the two are directly proportional. Substances that are fluorescent absorb light and then emit that light at a wavelength that is longer than the colorโs normal emitted wavelength range. In order to analyze this behavior, light is passed through the solution and then through a filter to be absorbed (and detected) by a sensor. Both the absorbed and emitted light are measured. Due to the method of detection, the compound (nanoparticules) must be fluorescent, translucent, and soluble.ย
First, a light source, such as a xenon lamp, emits light. The light passes through an excitation monochromator, which creates a specific wavelength known to excite the sample (I think). Then, the light passes through a lens before reaching the sample. The sample, placed inside a cuvette within a temperature-controlled box, absorbs the light and emits it. The emitted light is passed through a lens before being passed through an emission monochromator. Finally, the data is analyzed by a computer.
https://www.deltaopticalthinfilm.com/what-is-fluorescence-spectroscopy/
https://www.chromedia.org/chromedia?waxtrapp=mkqjtbEsHiemBpdmBlIEcCArB&subNav=cczbdbEsHiemBpdmBlIEcCArBP
https://microbiologyonlinenotes.com/fluorescence-spectroscopy-principle-instrumentation-and-application/
https://chem.libretexts.org/Bookshelves/Analytical_Chemistry/Supplemental_Modules_(Analytical_Chemistry)/Analytical_Sciences_Digital_Library/Active_Learning/In_Class_Activities/Molecular_and_Atomic_Spectroscopy/03_Text%3A_Molecular_and_Atomic_Spectroscopy/3%3A_Molecular_Luminescence/3.4%3A_Excitation_and_Emission_Spectra
