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Authored by  Joachim B

Adiponectin (ADPN) is an adipocyte-derived protein carrying anti-atherogenic properties in the general population. Controversially, ADPN serum concentrations are positively associated with the magnitude of chronic kidney disease (CKD), which is the strongest cardiovascular risk factor per se. This piece of reverse epidemiology raises the question, if ADPN is stimulated by uremia itself or is simply a bystander of CKD. To enlighten this question on the cellular level, we performed co-stimulatory experiments in human adipocytes using insulin- an established ADPN stimulating agent- and compound uremic haemofiltrate from patients with CKD stage 5. Haemofiltrate was employed as a model fluid because the more comprehensive approach of using uremic serum was methodologically impossible due to the ADPN content of serum.

Differentiated adipocytes were treated with compound uraemic haemofiltrate from patients with CKD stage 5 or PBS as control. Surprisingly, we did not detect any additional stimulatory effect of increasing haemofiltrate concentrations on adiponectin secretion of human adipocytes after 24 and 48 hours of incubation (p=0.67 or 0.07 resp.).

Therefore, this in vitro experiment preliminary precludes a pathophysiological effect of water-soluble uremic compounds on ADPN cellular secretion. Further experiments with varying amounts of insulin and other ADPN stimulators are needed to confirm such preclusion.

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