#simmon citrate

Citric acid (citrate) utilization by bacteria: Requirement, Procedure and Result

This test is used for differentiation of E. coli with Enterobacter aerogenes. It is a widely acceptable test for testing the presence of E. coli, a fecal organism in water which cannot utilize citrate as carbon source while E. aerogenes can use it. Secondly, citric acid is an intermediate of metabolic product of Kreb’s cycle which oxidizes pyruvate to CO2. The bacterium must have an ability to…