Glutenin, is consider the major storage proteins in the family of Poaceace which is also consider the main contents in wheat cereal of this family, to commercialize the ancient domesticated cereal like barley in the race of industrial crop and determining the uses of barley flour quality and resemblance of barley elite lines elasticity and dough quality, experimental assay was designed in the Laboratory of Plant Molecular Biology Department of Botany, university of Malakand on 100 elite lines of the barley.to detect High Molecular weight Glutenin protein extracted from the Barley seeds on the basis of Molecular weight, the Glu loci in barley 100 lines which score from the gel of polyacrylamide classify into nine different size (120, 105, 90, 80, 65, 45, 30, 20 and 10 KD) bands which further was classified into five different groups on the basis of variability present in selected barley lines and also in their Molecular weight; absence/presence and intensity of bands were observed. Largest group was observed is group 1 have 41% of elite lines in which 120, 105, 90, 80 and 65 KD molecular weight bands were recorded present. And some of the lines were found unknown which are not related any of these five group respectively, which clearly determine the novel allelic subunit and also show variation from the gene pool in these lines, further evaluation of these lines are needed to track these loci in this cash crop.
