Cuddles - Cardiologist/Cardiac Surgeon

Doom - Anesthesiologist/Cardiac Nurse

Livings - Mortician

Kitter - Soul Mortician

Meat - Hospital President

Snare - Charge Nurse

Shortnick - Pediatric Nurse

Party - Psychologist/Psychiatrist

Death - OBGYN/Neonatologist

Cheezeburger - Gastroenterologist

Kills - Pediatric Nurse

Suffuzz - Palliative Care Specialist

Peepbeep - Cafeteria Worker

Sunny - General/Emergency Ward GP Surgeon

Nya - General/Emergency Ward GP Surgeon

Lunesta - Pharmacist

Dino - Pharmacist

Memento - Radiologist

Essent - Assistant Radiology Technician

Ribbon - Gift Shop Dimension Owner/Clerk

Lizerd - Circus Ringleader

Pyro - Entertainer

Risk - Entertainer

Handerson - Psychiatric Assistant

Bonez - Orthopedist

Barry - Trans Male, Gay

Uni - Cis Male, Gay

Caroline - Non-Binary, Lesbian

Jay - Genderfluid, Bisexual

Hemera - Aroace

Doom - Transfeminine (fem-aligned non-binary), Gay

Cuddles - Trans Male, Gay

Kills - Trans Female, Lesbian

Snare - Cis Female, Lesbian

Shortnick - Cis Female

Livings - Cis Male

Suffuzz - Trans Male, Gay

Sunny - Non-Binary, Gay

Nya - Trans Female, Pansexual

Lunesta - Cis Female, Pansexual

Ribbon - Non-Binary, Pansexual

Meat - Trans Male, Gay

Doi 1 - Non-Binary

Doi 2 - Non-Binary

Handerson - Gay

Miley - Cis Female, Straight

Not outright confirmed but hinted at:

Livings - Definitely not straight (he’s married to a man)

Memento - Objectum

Cheezeburger - Objectum

Peepbeep - Male (unknown if trans or cis)

Also worth noting that Livings obviously isn’t straight because he’s married to a man, but a less well known one is that Memento is hinted at to be objectum because Kittycorn said they were married to their unrealistic x-ray machine. Dr. Cheezeburger is also hinted at to be objectum since his lollipop is labeled as his lover in his ref sheet. Peepbeep is also implied to be male ↓