Monoclonal Antibody Production is a comprehensive laboratory process that demonstrates how highly specific antibodies are generated using hybridoma technology. Students learn essential techniques, including sterile cell culture, mouse immunization, spleen cell isolation, myeloma cell fusion with polyethylene glycol (PEG), HAT selection, hybridoma cloning, FACS preparation, and ELISA-based antibody screening. The workflow also covers evaluating cell confluency, serial serum dilution, and hybridoma cryopreservation. By following each stage of monoclonal antibody production, learners gain a deeper understanding of antibody development, laboratory best practices, and the experimental methods widely used in biomedical research, diagnostics, and therapeutic antibody manufacturing.
Explore the monoclonal antibody production using hybridoma technology from immunization and cell fusion to ELISA screening in a virtual lab










