#phylogenetictree

Here is a step-by-step procedure of how Maca Pana created their phylogenetic tree:

1. Go to google chrome and search homologene. 

2. Type the disease in the search tab and download.

 3. At homologene downloader, pick the genomic sequences in fasta format and remove the repeated species and wait for it to download. 

4 . In mega, click edit/build alignment under align.

5. Under select an option, press create a new alignment. 

6. Press DNA when asked about the sequence alignment. 

7. Find the text earlier that was downloaded. 

8. Press alignment and align by M7: ClustaIW. 

9. Wait for the pairwise and multiple alignment to load and minimize this tab. 

10. Remove the excess genes from the ends based on the model organism. 

11. Press models at Mega and find the best dna/protein models and use the currently active data of phyloanalysis.meg, press compute and wait for it to load. 

12. Under phylogeny, press construct/test maximum likelihood tree and use the hasagawa kishino yano model + gamma as the model/method as it is the best model for the study. 

13. For the rates among sites, press gamma distributed and compute. 

14. This would then lead us to the original and bootstrap consensus tree

They used BLAST, which is an online app, and MEGA which is a software, to create the phylogenetic tree. Try it, too. 

CAN’T WAIT FOR THE BIG REVELATION! ALSO, WATCH THEIR NEXT EPISODE WHEREIN THEY WILL FEATURE TUTORIAL ABOUT THIS.