#phylogenetic study

Because of the high physical similarity within the Colletotrichum genus and the inaccuracy of traditional identification methods, genetic methods have become indispensable. Despite the genus Colletotrichum's tremendous variety, only a few morphological features are exhibited. The morphological investigation of Colletotrichum lupini and Colletotrichum acutatum isolates from olive trees and strawberries revealed colonies with the same look and colour, cylindrical conidia, and spindle-shaped conidia of various sizes. The ITS region, which contains the ITS1, ITS2 sections, and the 5,8S gene, was sequenced to identify the Colletotrichum isolates investigated. The OCol1, OCol2, OCol3, OCol4, and FCollu3 isolates had more than 95% similarity to Colletotrichum lupini while the OColA isolate had more than 95% similarity to Colletotrichum acutatum, according to a BLAST analysis of the acquired sequences with other sequences from GenBank. The phylogeny of the five isolates identified in this study allowed them to be divided into two groups: one containing four Moroccan isolates and another containing other isolates from various countries with a Moroccan isolate (accession number: MN064849), the latter's phylogenetic proximity to other isolates in GenBank being explained by.