Bio nanocomposite clay-based material Montmorillonite (MMT) and Titanium dioxide (TiO2) are modified for the application to antibacterial effect in waste water. TiO2 is employed because of its photostability, nontoxicity, high activity, and relatively low cost. In that view, the current study investigates the antibacterial potentials of TiO2/MMT nanocomposite formed by the hydrothermal synthesis route. The physicochemical properties of the formed TiO2/MMT nanocomposite were characterized by the powdered XRD, SEM, EDAX, BET, FTIR, and UV-Visible spectroscopies. The crystallinity and crystal structure are confirmed by the XRD, the bonding information and surface functionality by the FTIR, and the morphology and elemental composition with the SEM/EDAX analysis. From the BET investigations, the structural morphology of TiO2/MMT nanocomposite showed an improved pore volume, surface area, and voids, while the optical properties of TiO2 nanoparticles significantly enhanced following the composite formation with that of MMT. Further tests of antibacterial activity of TiO2/MMT nanocomposite towards Gram-positive (Bacillus cereus, Enterococcus and Staphylococcus aureus) and Gram-negative (Escheria coli and Pseudomonas aeruginosa) bacteria supported the efficiency of prepared composite and thereby confirming its promising potentials in the applications related to microbial removal from wastewater.
